Carbonic anhydrase is an ancient enzyme widespread in prokaryotes
Carbonic anhydrase is an ancient enzyme widespread in prokaryotes
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DOI：
10.1073/pnas.96.26.15184
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发表时间：
1999-12-21
影响因子：
11.1
通讯作者：
Ferry, JG
中科院分区：
文献类型：
--
作者：
Smith, KS;Jakubzick, C;Ferry, JG
碳酸酐酶催化CO2的可逆水合作用，并且在高度进化的真核生物中普遍存在。最近在甲烷古菌中鉴定出第三类碳酸酐酶（γ类），并且我们目前发现β类也从β结构域延伸到嗜热物种，这使我们开始在代谢和遗传多样性原核生物中系统地寻找这些酶。在这里，我们表明，碳酸酐酶是广泛存在于酵母和细菌领域，是一个古老的酶。在chemolithoautotrophic物种占据深分支的通用系统发育树的发生表明，这种酶的作用，在拟议的自养生活的起源。β和γ类的存在下，在代谢多样的物种跨越degreea和细菌域证明，碳酸酐酶有一个更广泛的和基本的作用，在原核生物学比以前认识到的。
Carbonic anhydrases catalyze the reversible hydration of CO2 and are ubiquitous in highly evolved eukaryotes, The recent identification of a third class of carbonic anhydrase (gamma class) in a methanoarchaeon and our present finding that the beta class also extends into thermophilic species from the Archaea domain led us to initiate a systematic search for these enzymes in metabolically and phylogenetically diverse prokaryotes. Here we show that carbonic anhydrase is widespread in the Archaea and Bacteria domains, and is an ancient enzyme. The occurrence in chemolithoautotrophic species occupying deep branches of the universal phylogenetic tree suggests a role for this enzyme in the proposed autotrophic origin of life. The presence of the beta and gamma classes in metabolically diverse species spanning the Archaea and Bacteria domains demonstrates that carbonic anhydrases have a far more extensive and fundamental role in prokaryotic biology than previously recognized.