Spine bone mineral density and vertebral body height are altered by alcohol consumption in growing male and female rats
Spine bone mineral density and vertebral body height are altered by alcohol consumption in growing male and female rats
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DOI：
10.1016/j.alcohol.2003.06.006
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发表时间：
2003-08-01
期刊：
影响因子：
2.3
通讯作者：
Callaci, JJ
中科院分区：
文献类型：
--
作者：
Wezeman, FH;Juknelis, D;Callaci, JJ
生长期的雄性和雌性大鼠长时间饮酒，会损害骨生成，减少骨的大小和质量。通常选择用于评价骨机械特性和结构特性、骨基质基因表达、生长因子组织浓度和骨矿物质密度（BMD）的实验动物骨骼部位为胫骨和股骨。很少有人关注脊柱和酒精对椎体BMD和椎体高度的影响。将15只雄性和15只雌性Sprague-Dawley大鼠（30日龄）分为3组：酒精喂养组，与同性别动物配对喂养的非酒精等热量喂养对照组相匹配，以及自由喂养对照组。酒精喂养的动物接受Lieber-DeCarli液体饮食，其中含有36%的热量摄入酒精;等热量配对喂养的大鼠接受相同的饮食，但不含酒精。喂养45天后，取出腰椎。从每个脊柱解剖第四腰椎，并测量椎体高度。与任一对照组的结果相比，雄性和雌性大鼠的腰椎椎体高度均因饮酒而显著降低。通过外周定量计算机断层扫描（pQCT）确定椎体的松质骨密度和皮质骨密度。自由采食组的雄性和雌性大鼠（75日龄）椎体皮质骨和松质骨BMD相似，皮质骨BMD大于松质骨BMD。腰椎椎体松质骨和皮质骨密度下降的雄性和雌性大鼠响应饮酒45天相比，任何对照组的结果。慢性饮酒后，男性和女性松质骨的BMD损失均大于皮质骨。生长期大鼠长期饮酒导致椎骨生长减慢和椎骨骨质减少。(C)2003年爱思唯尔公司All rights reserved.
Alcohol, consumed for extended periods by growing male and female rats, impairs osteogenesis and reduces bone size and mass. The skeletal sites of experimental animals commonly chosen for an evaluation of bone mechanical characteristics and architectural properties, bone matrix gene expression, tissue concentrations of growth factors, and bone mineral density (BMD) have been the tibiae and femora. Far less attention has been focused on the spine and the effects of alcohol on vertebral BMD and vertebral body height. Fifteen male and 15 female Sprague-Dawley rats (aged 30 days) were divided into three groups: an alcohol-fed group, matched to a pair-fed non-alcohol isocaloric-fed control group with animals of the same sex, and an ad libitum-fed control group. Alcohol-fed animals received a Lieber-DeCarli liquid diet containing 36% of caloric intake as alcohol; isocaloric pair-fed rats received the same diet without alcohol. After 45 days of feeding, the lumbar spine was removed. The fourth lumbar vertebra from each spine was dissected, and the vertebral body height was measured. Lumbar vertebral body height was significantly reduced by alcohol consumption in both male and female rats compared with findings for either control group. Cancellous and cortical BMD of the vertebral body was determined by peripheral quantitative computed tomography (pQCT). Male and female rats (aged 75 days) in the ad libitum-fed group had similar vertebral body cortical and cancellous BMD, with cortical BMD being greater than cancellous BMD. Lumbar vertebral body cancellous and cortical BMD declined for both male and female rats in response to alcohol consumption for 45 days compared with findings for either control group. More BMD loss occurred from cancellous than from cortical bone in both sexes after chronic alcohol consumption. Chronic alcohol consumption by growing rats results in vertebral growth reduction and vertebral osteopenia. (C) 2003 Elsevier Inc. All rights reserved.