Cutting edge:: The common γ-chain is an indispensable subunit of the IL-21 receptor complex
Cutting edge:: The common γ-chain is an indispensable subunit of the IL-21 receptor complex
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DOI：
10.4049/jimmunol.167.1.1
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发表时间：
2001-07-01
影响因子：
4.4
通讯作者：
Sugamura, K
中科院分区：
文献类型：
--
作者：
Asao, H;Okuyama, C;Sugamura, K
常见的伽马链 (gamma (c)) 是 IL-4、IL-7、IL-9、IL-15 以及 IL-2 功能性受体复合物不可或缺的亚基。在这里，我们表明伽玛 (c) 也与 IL-21R 复合物共享。尽管IL-21与γ(c)缺陷型ED40515(-)细胞上表达的IL-21R结合，但IL-21不能转导任何胞质内信号。然而，在 ED gamma -16 细胞（γ (c) 转染的 ED40515-细胞系）中，IL-21 与 IL-21R 结合，并可激活 Janus 激酶 (JAK)I、JAK3、STAT1 和 STAT3。化学交联研究揭示了 IL-21 与 γ 的直接结合 (c)。这些数据清楚地表明，γ (c) 是功能性 IL-21R 复合物不可或缺的亚基。
The common gamma -chain (gamma (c)) is an indispensable subunit of the functional receptor complexes for IL-4, IL-7, IL-9, and IL-15 as well as IL-2. Here we show that the gamma (c) is also shared with the IL-21R complex. Although IL-21 binds to the IL-21R expressed on gamma (c)-deficient ED40515(-) cells, IL-21 is unable to transduce any intracytoplasmic signals. However, in ED gamma -16 cells, a gamma (c)-transfected ED40515- cell line, IL-21 binds to the IL-21R and can activate Janus kinase (JAK)I, JAK3, STAT1, and STAT3. The chemical cross-linking study reveals the direct binding of IL-21 to the gamma (c). These data clearly demonstrate that the gamma (c) is an indispensable subunit of the functional IL-21R complex.