Whole-cell catalytic synthesis of 2-O-α-glucopyranosyl-l-ascorbic acid by sucrose phosphorylase from Bifidobacterium breve via a batch-feeding strategy
Whole-cell catalytic synthesis of 2-O-α-glucopyranosyl-l-ascorbic acid by sucrose phosphorylase from Bifidobacterium breve via a batch-feeding strategy
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短双歧杆菌蔗糖磷酸化酶通过分批补料策略全细胞催化合成 2-O-α-吡喃葡萄糖基-l-抗坏血酸
DOI：
10.1016/j.procbio.2021.11.023
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发表时间：
2022-01
影响因子：
4.4
通讯作者：
Xiaolong Chen
中科院分区：
文献类型：
--
作者：
Yaoyao Zhou;Gan Tian;Ruini Jiang;Hanchi Chen;Zhi Ma;Yuele Lu;Zhu Linjiang;Xiaolong Chen
l -抗坏血酸2-O-α- d -葡萄糖苷（AA-2G）是一种高度稳定的维生素C衍生物，广泛应用于化妆品、食品和制药等领域。蔗糖磷酸化酶（SPase）最近被报道对产生AA-2G有效。本研究发现，来自短双歧杆菌的SPase （BbrSPase）具有高活性和良好的热稳定性，是一种新的aa糖基化工具。AA糖基化的最佳条件为pH 5.5和55℃。蔗糖作为糖基供体时，K m和K c a t分别为2.72±0.49 mM和9.70±0.10 s−1。果糖严重抑制了转糖基化活性，导致糖基化速率急剧下降，但由于对蔗糖的高亲和力，并不一定阻止AA-2G的产率。因此，为了减轻果糖的抑制作用，研究人员开发了一种新的蔗糖分批进料策略，以有效地生产AA-2G。在72 h内最大滴度为185 g/L， L -AA的摩尔转化率达到50.2%，是我们所知的最高转化率。
Abstract l -ascorbic acid 2-O-α- d -glucoside (AA-2G), a highly stabilized vitamin C derivative, has been widely applied in cosmetics, foods, and pharmaceuticals. Sucrose phosphorylase (SPase) was recently reported to be effective for producing AA-2G. In this study, SPase from Bifidobacterium breve (BbrSPase) was identified as a new AA-glycosylating tool with high activity and good thermostability. The optimal conditions for AA glycosylation are pH 5.5 and 55 °C. The K m and k c a t when sucrose was applied as a glycosyl donor were 2.72 ± 0.49 mM and 9.70 ± 0.10 s−1, respectively. The transglycosylation activity was seriously inhibited by fructose, which caused a sharp decline in the glycosylation rate but did not necessarily stop the yield of AA-2G due to the high affinity to sucrose. Therefore, to attenuate the inhibition of fructose, a new sucrose batch-feeding strategy was developed for the effective production of AA-2G. The maximum titer was 185 g/L within 72 h. The molar conversion rate of l -AA reached 50.2 %, which is the highest conversion rate to our knowledge.
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影响因子：
2.7
作者：
Lee, Jin-Ha;Moon, Young-Hwan;Kim, Doman
通讯作者：
Kim, Doman
影响因子：
2.7
作者：
N. Muto;Tomoe Y. Nakamura;I. Yamamoto
通讯作者：
N. Muto;Tomoe Y. Nakamura;I. Yamamoto
影响因子：
1.6
作者：
KITAO, S;ARIGA, T;SEKINE, H
通讯作者：
SEKINE, H
DOI：
10.1271/bbb.58.419
发表时间：
1994-02
期刊：
Bioscience, Biotechnology, and Biochemistry
影响因子：
--
作者：
S. Kitao;H. Sekine
通讯作者：
S. Kitao;H. Sekine
影响因子：
1.8
作者：
Goedl, Christiane;Sawangwan, Thornthan;Nidetzky, Bernd
通讯作者：
Nidetzky, Bernd