Pan-keratin Immunostaining in Human Tumors: A Tissue Microarray Study of 15,940 Tumors.
Pan-keratin Immunostaining in Human Tumors: A Tissue Microarray Study of 15,940 Tumors.
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DOI：
10.1177/10668969221117243
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发表时间：
2023-09
影响因子：
1.2
通讯作者：
Bernreuther, Christian
中科院分区：
文献类型：
--
作者：
Menz, Anne;Gorbokon, Natalia;Viehweger, Florian;Lennartz, Maximilian;Hube-Magg, Claudia;Hornsteiner, Lisa;Kluth, Martina;Voelkel, Cosima;Luebke, Andreas M.;Fraune, Christoph;Uhlig, Ria;Minner, Sarah;Dum, David;Hoeflmayer, Doris;Sauter, Guido;Simon, Ronald;Burandt, Eike;Clauditz, Till S.;Lebok, Patrick;Jacobsen, Frank;Steurer, Stefan;Krech, Till;Marx, Andreas H.;Bernreuther, Christian
为评价泛角蛋白免疫染色的有效性，应用免疫组织化学方法对121例不同肿瘤类型和亚型的13,501例肿瘤标本和76种不同正常组织类型的608例肿瘤标本进行了组织芯片分析。在正常组织中，泛角蛋白免疫组织化学染色主要见于上皮细胞。肝细胞、朗格汉斯胰岛和肺泡细胞的染色强度较低，而肾上腺皮质的染色强度明显减弱。在75个上皮性肿瘤实体中，包括几乎所有腺癌、鳞状细胞癌和尿路上皮癌在内的62例(83%)标本中，泛角蛋白在≥中的阳性率为98%。121个肿瘤实体中只有17个(13%)的泛角蛋白阳性率在25%到98%之间，包括混合分化肿瘤、内分泌/神经内分泌肿瘤、肾细胞癌、肾上腺皮质肿瘤和特别低分化的癌亚型。泛角蛋白阳性在0.9%~25%的15个实体大多为间充质来源。泛角蛋白免疫染色减少/缺失与高UICC期(p = 0.0001)、高Thoenes分级(p = 0.0183)、高Fuhrman分级(p = 0.0049)、晚期肿瘤(p 和lt； 0.0001)和淋巴结转移(p = 0.0114)，乳头状肾细胞癌进展期PT期(p = 0.0007)，无特殊类型浸润性乳腺癌ER和PR表达缺失(p = 和p = 0.0005)，晚期(p 0.0023)和高分级(p 0.0005)。综上所述，尽管泛角蛋白在肾细胞、肾上腺皮质和神经内分泌肿瘤中的一小部分呈阴性，但在绝大多数上皮性肿瘤中都能持续检测到泛角蛋白。这些数据还将泛角蛋白免疫染色减少与上皮性肿瘤中不利的肿瘤表型联系起来。
To evaluate the efficiency of pan-keratin immunostaining, tissue microarrays of 13,501 tumor samples from 121 different tumor types and subtypes as well as 608 samples of 76 different normal tissue types were analyzed by immunohistochemistry. In normal tissues, strong pan-keratin immunostaining was seen in epithelial cells. Staining intensity was lower in hepatocytes, islets of Langerhans, and pneumocytes but markedly reduced in the adrenal cortex. Pan-keratin was positive in ≥98% of samples in 62 (83%) of 75 epithelial tumor entities, including almost all adenocarcinomas, squamous cell and urothelial carcinomas. Only 17 of 121 tumor entities (13%) had a pan-keratin positivity rate between 25% and 98%, including tumors with mixed differentiation, endocrine/neuroendocrine tumors, renal cell carcinomas, adrenocortical tumors, and particularly poorly differentiated carcinoma subtypes. The 15 entities with pan-keratin positivity in 0.9%-25% were mostly of mesenchymal origin. Reduced/absent pan-keratin immunostaining was associated with high UICC stage (p = 0.0001), high Thoenes grade (p = 0.0183), high Fuhrman grade (p = 0.0049), advanced tumor stage (p < 0.0001) and lymph node metastasis (p = 0.0114) in clear cell renal cell carcinoma, advanced pT stage (p = 0.0007) in papillary renal cell carcinoma, and with advanced stage (p = 0.0023), high grade (p = 0.0005) as well as loss of ER and PR expression (each p < 0.0001) in invasive breast carcinoma of no special type (NST). In summary, pan-keratin can consistently be detected in the vast majority of epithelial tumors, although pan-keratin can be negative a fraction of renal cell, adrenocortical and neuroendocrine neoplasms. The data also link reduced pan-keratin immunostaining to unfavorable tumor phenotype in in epithelial neoplasms.
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影响因子：
5.6
作者：
Matoso, Andres;Epstein, Jonathan I.
通讯作者：
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