Evidence That Vibrio vulnificus AhpC2 Is Essential for Survival Under High Salinity by Modulating Intracellular Level of ROS
Evidence That Vibrio vulnificus AhpC2 Is Essential for Survival Under High Salinity by Modulating Intracellular Level of ROS
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DOI：
10.1007/s12275-009-0227-2
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发表时间：
2010-02-01
影响因子：
3
通讯作者：
Choi, Sang Ho
中科院分区：
文献类型：
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作者：
Koh, Myung Jin;Lee, Hyun Sung;Choi, Sang Ho
编码创伤弧菌（一种食源性病原体）的烷基过氧化氢还原酶的ahpC2的表达根据培养物中的NaCl浓度而递增诱导。在高盐条件下，ahpC2突变体的生长受到显著影响，滞后期延长，生长速率降低。在高盐胁迫下，创伤弧菌细胞内ROS大量积累，其中ahpC2突变体积累的ROS水平高于亲本野生型。因此，综合结果表明，AhpC2通过清除细胞中的ROS有助于创伤弧菌在高盐度下的生长。
Expression of ahpC2 encoding an alkyl hydroperoxide reductase of Vibrio vulnificus, a foodborne pathogen, was incrementally induced depending on NaCl concentrations in the culture. Growth of the ahpC2 mutant was significantly impaired with longer lag phase and lower growth rate when cultured under high salinity. ROS was accumulated in V. vulnificus cells when stressed by exposure to high salinity, and the ahpC2 mutant accumulated higher level of ROS as compared with the parental wild type. Consequently, the combined results suggest that AhpC2 contributes to the growth of V. vulnificus under high salinity by scavenging ROS in cells.