Translocation Behaviors of Synthetic Polyelectrolytes through Alpha-Hemolysin (α-HL) and Mycobacterium smegmatis Porin A (MspA) Nanopores
Translocation Behaviors of Synthetic Polyelectrolytes through Alpha-Hemolysin (α-HL) and Mycobacterium smegmatis Porin A (MspA) Nanopores
复制标题
合成聚电解质通过α-溶血素 (α-HL) 和耻垢分枝杆菌孔蛋白 A (MspA) 纳米孔的易位行为
DOI：
10.1149/1945-7111/ac6c55
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发表时间：
2022
影响因子：
3.9
通讯作者：
Liu, Chang
中科院分区：
文献类型：
--
作者：
Wang, Xiaoqin;Stevens, Kaden C.;Ting, Jeffrey M.;Marras, Alexander E.;Rezvan, Gelareh;Wei, Xiaojun;Taheri-Qazvini, Nader;Tirrell, Matthew V.;Liu, Chang
由于其负磷酸主链，dna已被用作非带电生物大分子的纳米孔传感探针。受此启发，我们通过研究PEO-b-PSS和PEO-b-PVBTMA在常用的α-溶血素（α-HL）和耻毛杆菌孔蛋白A （MspA）纳米孔中的易位行为，探索了双块合成聚电解质作为更灵活、更廉价的纳米孔传感探针的潜力。不同孔隙取向和测试电压下的易位记录表明，PEO-b-PSS通过α-HL易位，PEO-b-PVBTMA通过MspA易位。这项工作提供了深入了解合成聚电解质为基础的探针，以扩大探针的选择和灵活性的纳米孔传感。
DNAs have been used as probes for nanopore sensing of noncharged biomacromolecules due to its negative phosphate backbone. Inspired by this, we explored the potential of diblock synthetic polyelectrolytes as more flexible and inexpensive nanopore sensing probes by investigating translocation behaviors of PEO-b-PSS and PEO-b-PVBTMA through commonly used alpha-hemolysin (α-HL) and Mycobacterium smegmatis porin A (MspA) nanopores. Translocation recordings in different configurations of pore orientation and testing voltage indicated efficient PEO-b-PSS translocations through α-HL and PEO-b-PVBTMA translocations through MspA. This work provides insight into synthetic polyelectrolyte-based probes to expand probe selection and flexibility for nanopore sensing.