SEQUENCE SPECIFIC CLEAVAGE OF DNA BY MICROCOCCAL NUCLEASE
SEQUENCE SPECIFIC CLEAVAGE OF DNA BY MICROCOCCAL NUCLEASE
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DOI：
10.1093/nar/9.12.2643
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
ALTENBURGER, W
中科院分区：
文献类型：
--
作者：
HORZ, W;ALTENBURGER, W
微球菌核酸酶显示在部分消化条件下以特异性方式切割DNA。优先攻击5′CATA和5′CTA型序列，然后在新产生的DNA末端进行外切Tytic降解。富含GC的侧翼序列进一步增加了初始攻击的可能性。出乎意料的是，只含有A和T的长片段被核酸酶保留下来。用小鼠卫星DNA和质粒pBR 322的两个片段获得的这些结果不支持先前的论点，即最初被微球菌核酸酶切割的是AT含量高的区域。微球菌核酸酶的这种特异性使其在旨在监测染色质中DNA序列的核蛋白结构的实验中的应用复杂化。
Micrococcal nuclease is shown to cleave DNA under conditions of partial digestion in a specific manner. Sequences of the type 5′CATA and 5′CTA are attacked preferentially, followed by exonucleoTytic degradation at the newly generated DNA termini. GC-rich flanking sequences further increase the probability of initial attack. Unexpectedly, long stretches containing only A and T are spared by the nuclease. These results, which were obtained with mouse satellite DNA and two fragments from the plasmid pBR322, do not support the previous contention that it is the regions of high AT-content which are initially cleaved by micrococcal nuclease. This specificity of micrococcal nuclease complicates its use in experiments intended to monitor the nucleoprotein structure of a DNA sequence in chromatin.