Physiological tolerance of marine calanoid copepod eggs to sulphide
Physiological tolerance of marine calanoid copepod eggs to sulphide
复制标题
DOI：
10.3354/meps328171
复制
发表时间：
2006-12
影响因子：
2.5
通讯作者：
Pernille Nielsen;J. Mortensen;B. Vismann;B. Hansen
中科院分区：
文献类型：
--
作者：
Pernille Nielsen;J. Mortensen;B. Vismann;B. Hansen
研究了缺氧和缺氧-硫化物（8种不同的硫化物浓度，范围从10 μmol l-1到10 mmol l-1）对浮游桡足类Acartia tonsa Dana的次幼体卵孵化的影响。因此，实验在pH 8.1下进行。将实验室饲养的样品所产的亚致死卵暴露于缺氧和缺氧-硫化物中不同时间（3、7、14、30、60、120、180和240 d），然后转移到常氧条件下。短期（3至60天）暴露于缺氧或缺氧-硫化物并没有显着影响孵化成功的鸡蛋，但孵化一般随着暴露时间的增加而下降。暴露60 d后，缺氧和缺氧-硫化物（硫化物浓度≥250 μmol l-1）引起的效应有显著差异。暴露240 d后，缺氧处理和缺氧-硫化物（所有硫化物浓度）处理的卵的孵化率有显著差异。在一项短期实验中，将亚硝化卵同时暴露在氧气和不同浓度的硫化物中，结果表明硫化物能够穿过蛋壳。暴露于常氧条件下的鸡蛋代谢率为1.86 ± 0.57 µJ h-1。暴露于缺氧的鸡蛋的代谢率为0.08 ± 0.02 µJ h-1，而暴露于缺氧和14.7 mmol l-1硫化物的鸡蛋的代谢率为0.25 ± 0.001 µJ h-1。根据代谢实验和卵内pH值约为6的事实，提出了一种未知的硫化物防御机制。通萨鸡蛋。
The impact of anoxia and anoxia-sulphide (8 different numeric sulphide concentrations, ranging from 10 µmol l -1 to 10 mmol l -1 ) on hatching of subitaneous eggs of the planktonic copepod Acartia tonsa Dana was evaluated. The experiments were consequently conducted at pH 8.1. Subita- neous eggs, spawned by laboratory-reared specimens, were exposed to anoxia and anoxia-sulphide for different periods (3, 7, 14, 30, 60, 120, 180 and 240 d) and then transferred to normoxic conditions. Short-term (3 to 60 d) exposure to anoxia or anoxia-sulphide did not significantly affect the hatching success of the eggs, but hatching generally declined with increasing length of exposure. After 60 d of exposure there were significant differences between the effects caused by anoxia and anoxia-sul- phide (sulphide concentrations ≥250 µmol l -1 ). After 240 d of exposure there were significant differ- ences in hatching between eggs treated with anoxia and those with anoxia-sulphide (all sulphide concentrations). A short-term experiment, where subitaneous eggs were simultaneously exposed to oxygen and different sulphide concentrations, indicated that sulphide is capable of crossing the eggshell. The metabolic rate of eggs exposed to normoxic conditions was 1.86 ± 0.57 µJ h -1 . Eggs exposed to anoxia had a metabolic rate of 0.08 ± 0.02 µJ h -1 , whereas eggs exposed to anoxia and 14.7 mmol l -1 sulphide had a metabolic rate of 0.25 ± 0.001 µJ h -1 . Based on the metabolism ex- periments and the fact that internal egg pH was ~6, an unknown sulphide defence mechanism is suggested to be present in A. tonsa eggs.