QUANTITATIVE DETERMINATION OF PHOSPHATIDAL ETHANOLAMINE AND OTHER PHOSPHATIDES IN VARIOUS TISSUES OF RAT
QUANTITATIVE DETERMINATION OF PHOSPHATIDAL ETHANOLAMINE AND OTHER PHOSPHATIDES IN VARIOUS TISSUES OF RAT
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DOI：
10.1016/0003-2697(68)90246-7
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发表时间：
1968-01-01
影响因子：
2.9
通讯作者：
TANAKA, T
中科院分区：
文献类型：
--
作者：
KEENAN, RW;SCHMIDT, G;TANAKA, T
基于薄层色谱法的方法被设计用于测定微克量的磷脂。利用这些化合物的[α]，[β] -不饱和醚键在汞盐存在下水解的事实，使用该程序估计乙醇胺缩醛磷脂[在大鼠脑、肺、脾、肌肉、子宫、肾、胰腺、心脏、肝脏中]的量和放射性。研究该反应的条件以确保发生完全水解并且酯磷脂不受影响。在这些条件下，估计溶血磷脂酰乙醇胺生产允许准确定量的磷脂乙醇胺的脂质混合物。用这种方法获得的值的磷脂组成的整个大鼠脑，和磷脂乙醇胺含量的一些其他组织。该测定方法也可应用于其他色谱系统。
A method based on thin-layer chromatography was devised for the determination of microgram quantities of the phospholipids. This procedure was used to estimate both the quantity and the radioactivity of the ethanolamine plasmalogens [in rat brain, lung, spleen, muscle, uterus, kidney, pancreas, heart, liver] by taking advantage of the fact that the [alpha],[beta] -unsaturated ether bond of these compounds is hydrolyzed in the presence of mercury salts. The conditions of this reaction were investigated to ensure that complete hydrolysis took place and that the ester phospholipids were not affected. Under these conditions, estimation of the lysophosphatidyl ethanolamine produced permitted accurate quantitation of the phosphatidal ethanolamine in the lipid mixture. With this method values were obtained for the phosphatide composition of whole rat brain, and the phosphatidal ethanolamine content of a number of other tissues. The assay procedure can also be applied to other chromatographic systems.