Keratinocyte-derived T-cell growth factor: a T-cell growth factor functionally distinct from interleukin 2.
Keratinocyte-derived T-cell growth factor: a T-cell growth factor functionally distinct from interleukin 2.
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角质形成细胞衍生的 T 细胞生长因子：功能上不同于白细胞介素 2 的 T 细胞生长因子。
DOI：
10.1073/pnas.83.12.4451
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发表时间：
1986
影响因子：
11.1
通讯作者：
Horowitz,MC
中科院分区：
文献类型：
--
作者：
Kupper,TS;Coleman,DL;McGuire,J;Goldminz,D;Horowitz,MC
T细胞生长因子，最近被称为白细胞介素2（IL-2），是活化的T淋巴细胞的产物，并且被认为是T淋巴细胞的主要营养因子。IL-2制剂的活性通过它们支持各种IL-2依赖性细胞系生长的程度来评估。我们报告说，小鼠表皮上皮细胞（角质形成细胞）产生并释放一种因子，支持辅助T细胞衍生的IL-2依赖性细胞系HT-2的生长。这种物质，角化细胞衍生的T细胞生长因子（KTGF），不支持来源于细胞毒性T细胞的IL-2依赖性细胞系（CTLL-2系）的生长。这种对IL-2依赖性细胞系的差异效应是KTGF所独有的。KTGF具有25，000 - 35，000的表观分子量，并且通过反相和凝胶过滤HPLC分析具有与常规IL-2相似的性质。然而，即使是高度纯化的KTGF也不能刺激CTLL-2细胞的增殖。观察到表皮上皮细胞产生T淋巴细胞的营养因子，这可能有助于解释在某些皮肤病中皮肤微环境中T细胞优先增殖的基础。此外，这表明不同的IL-2依赖性T细胞系可能具有不同的生长要求，并且非淋巴细胞类型可能产生能够维持T细胞生长的因子。
T-cell growth factor, more recently termed interleukin 2 (IL-2), is the product of activated T lymphocytes and is considered the principal trophic factor for T lymphocytes. The activity of IL-2 preparations is assessed by the degree to which they support the growth of various IL-2-dependent cell lines. We report that murine epidermal epithelial cells (keratinocytes) produce and release a factor that supports the growth of the helper-T-cell-derived, IL-2-dependent cell line HT-2. This substance, keratinocyte-derived T-cell growth factor (KTGF), does not support the growth of an IL-2-dependent cell line derived from cytotoxic T cells (line CTLL-2). This differential effect on IL-2-dependent cell lines is unique to KTGF. KTGF has an apparent molecular weight of 25,000-35,000 and has properties similar to those of conventional IL-2 by reversed-phase and gel-filtration HPLC analysis. However, even highly purified KTGF fails to stimulate the proliferation of CTLL-2 cells. The observation that epidermal epithelium produces a trophic factor for T lymphocytes may help explain the basis for preferential proliferation of T cells in the microenvironment of skin in certain dermatologic disorders. Further, it suggests that different IL-2-dependent T-cell lines may have distinct growth requirements and that non-lymphocyte cell types may produce factors capable of maintaining the growth of T cells.