Morphometric analysis of human bone biopsies:: A quantitative structural comparison of histological sections and micro-computed tomography
Morphometric analysis of human bone biopsies:: A quantitative structural comparison of histological sections and micro-computed tomography
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DOI：
10.1016/s8756-3282(98)00068-4
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发表时间：
1998-07-01
期刊：
影响因子：
4.1
通讯作者：
Rüegsegger, P
中科院分区：
文献类型：
--
作者：
Müller, R;Van Campenhout, H;Rüegsegger, P
作为欧盟BIOMED I研究“骨质疏松症骨质量评估”的一部分，通过常规组织形态学和三维显微计算机断层扫描（mu CT）评估了髂间骨活检的小梁结构。本研究从27名女性和36名男性（年龄23-92岁）死后获得63个圆柱形人髂骨标本，并进行形态计量学评价。对于传统的组织形态计量学分析，从骨芯的中外侧平面的两个区域制备一对四个不脱钙切片。用von Kossa技术对切片进行染色，以获得高对比度的二维图像。在组织切片之前，用台式mu CT测量样品，提供14 μ m的标称分辨率。两种方法计算的二维和三维形态学参数分别为骨体积密度（BV/TV）、骨表面密度（BS/TV）、骨小梁厚度（Tb，Th）和骨小梁间距（Tb，Sp），结果均具有高度显著性BV/TV的常规组织学和mu CT之间的相关性（p < 0.0001）（r = 0.93），BS/TV（r = 0.91），Tb.Th（r = 0.84），与Tb.Sp（r = 0.91），组织学和I.ECT之间的平均百分比差异范围为2.5%（BV/TV）至6.1%（Tb，Th），传统的组织形态测量和微断层摄影分析之间的高相关性和低差异的绝对数是非常有前途的微断层摄影成像的使用。mu CT是一种非破坏性、快速且非常精确的程序，允许测量未处理活检或小骨中的松质骨和密质骨，以及全自动确定三维形态测量指标。(Bone 23：59-66; 1998）（C）1998由Elsevier Science Inc. All rights reserved.
As a part of the European Union BIOMED I study "Assessment of Bone Quality in Osteoporosis," the trabecular structure of transiliac bone biopsies was assessed by conventional histomorphometry and by three-dimensional micro-computed tomography (mu CT). Sixty-three cylindrical human transiliac bone specimens were obtained post mortem from 27 women and 36 men (age 23-92 years) and evaluated morphometrically in this study, For the conventional histomorphometric analysis, a pair of four undecalcified sections were prepared from two regions in the mediolateral plane of the bone cores. The sections were stained with the von Kossa technique to obtain high-contrast two-dimensional images. Prior to the histologic sectioning the samples were measured with a desktop mu CT, providing a 14 mu m nominal resolution. The morphometric parameters computed by both methods in two or three dimensions, respectively, were bone volume density (BV/TV), bone surface density (BS/TV), trabecular thickness (Tb,Th), and trabecular separation (Tb,Sp), Results showed highly significant (p < 0.0001) correlations between conventional histology and mu CT for BV/TV (r = 0,93), BS/TV (r = 0.91), Tb.Th (r = 0.84), and Tb.Sp (r = 0,91), The mean percentage differences between histology and I.ECT ranged from 2.5% (BV/TV) to 6.1% (Tb,Th), The high correlations and the low differences in terms of absolute numbers between conventional histomorphometric and micro-tomographic analysis are very promising for the use of micro-tomographic imaging. mu CT is a nondestructive, fast, and very precise procedure that allows the measurement of cancellous and compact bone in unprocessed biopsies or small bones, as well as a fully automatic determination of three-dimensional morphometric indices. (Bone 23:59-66; 1998) (C) 1998 by Elsevier Science Inc. All rights reserved.