Blocking BE301622 gene expression by RNAi initiates differentiation of neural stem cells in rat
Blocking BE301622 gene expression by RNAi initiates differentiation of neural stem cells in rat
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通过 RNAi 阻断 BE301622 基因表达可启动大鼠神经干细胞的分化
DOI：
10.1002/cbf.1367
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发表时间：
2007-11-01
影响因子：
3.6
通讯作者：
Li, Hailong
中科院分区：
文献类型：
--
作者：
Wen, Tieqiao;Bao, Kuiyi;Li, Hailong
Neural stem cells (NSCs) are capable of differentiating into neurons, astrocytes and oligodendrocytes. However, the molecular mechanisms regulating NSCs differentiation are not well understood. Our previous research by microarray analysis certified that a lot of genes are differentially expressed in the course of NSC differentiation. In this study we report the function of one of these genes, BE301622, by RNAi techniques. To silence the BE301622 gene, a long, double‐stranded RNA (dsRNA) was synthesized by using a kit (Ambion T7 MegaScript) and transformed into NSCs. Expression of mRNA was tested through RT‐PCR. The result showed the expression of BE301622 gene was specificially suppressed. This finding effectively validated that BE301622 is involved in the differentiation of NSCs. Copyright © 2006 John Wiley & Sons, Ltd.
