Saccharomyces cerevisiae S288C genome annotation:: a working hypothesis
Saccharomyces cerevisiae S288C genome annotation:: a working hypothesis
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DOI：
10.1002/yea.1400
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发表时间：
2006-09-01
期刊：
影响因子：
2.6
通讯作者：
Cherry, J. Michael
中科院分区：
文献类型：
--
作者：
Fisk, Dianna G.;Ball, Catherine A.;Cherry, J. Michael
酿酒酵母基因组是特征最清楚的真核基因组，也是识别开放阅读框 (ORF) 方面最简单的基因组之一，但其主要注释自 1996 年首次发布以来的十年中不断更新（Goffeau 等，1996）。酵母基因组数据库（SGD；www.yeastgenome.org）（Hirschman 等，2006）是该参考基因组的社区指定存储库，致力于确保酿酒酵母注释尽可能准确和有用。在 SGD，酿酒酵母基因组序列和注释被视为工作假设，必须反复测试和完善。在本文中，为了庆祝酿酒酵母基因组序列完成十周年，我们讨论了酿酒酵母序列和注释的变化方式，考虑了这些变化所基于的实验和比较数据的多种来源，并描述了我们评估、整合和记录这些新数据的方法。版权所有 (c) 2006 John Wiley & Sons, Ltd.
The S. cerevisiae genome is the most well-characterized eukaryotic genome and one of the simplest in terms of identifying open reading frames (ORFs), yet its primary annotation has been updated continually in the decade since its initial release in 1996 (Goffeau et al., 1996). The Saccharomyces Genome Database (SGD; www.yeastgenome.org) (Hirschman et al., 2006), the community-designated repository for this reference genome, strives to ensure that the S. cerevisiae annotation is as accurate and useful as possible. At SGD, the S. cerevisiae genome sequence and annotation are treated as a working hypothesis, which must be repeatedly tested and refined. In this paper, in celebration of the tenth anniversary of the completion of the S. cerevisiae genome sequence, we discuss the ways in which the S. cerevisiae sequence and annotation have changed, consider the multiple sources of experimental and comparative data on which these changes are based, and describe our methods for evaluating, incorporating and documenting these new data. Copyright (c) 2006 John Wiley & Sons, Ltd.