Activated RSC-nucleosome complex and persistently altered form of the nucleosome
Activated RSC-nucleosome complex and persistently altered form of the nucleosome
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DOI：
10.1016/s0092-8674(00)81218-0
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发表时间：
1998-07-10
期刊：
影响因子：
64.5
通讯作者：
Kornberg, RD
中科院分区：
文献类型：
--
作者：
Lorch, Y;Cairns, BR;Kornberg, RD
RSC是一种与SWI/SNF复合物相关的丰富的、必需的染色质重塑复合物，其结合核小体和裸DNA的亲和力相当，如凝胶位移分析所示。RSC-核小体复合物在ATP存在下转化为较慢的迁移形式。这种激活的复合物表现出对内切酶和外切酶的敏感性大大增加，但保留了组蛋白的完整补充。激活持续在ATP的情况下，并在RSC的去除，核小体被释放在一个改变的形式，减少电泳迁移率，更大的沉降速率，并在离子强度升高显着不稳定。该反应在RSC和ATP存在下是可逆的，改变的形式转化回核小体。
RSC, an abundant, essential chromatin-remodeling complex, related to SWI/SNF complex, binds nucleosomes and naked DNA with comparable affinities, as shown by gel shift analysis. The RSC-nucleosome complex is converted in the presence of ATP to a slower migrating form. This activated complex exhibits greatly increased susceptibility to endo- and exonucleases but retains a full complement of histones. Activation persists in the absence of ATP, and on removal of RSC, the nucleosome is released in an altered form, with a diminished electrophoretic mobility, greater sedimentation rate, and marked instability at elevated ionic strength. The reaction is reversible in the presence of RSC and ATP, with conversion of the altered form back to the nucleosome.