Expression profiling of DNA repair genes in human oocytes and blastocysts using microarrays
Expression profiling of DNA repair genes in human oocytes and blastocysts using microarrays
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DOI：
10.1093/humrep/dep224
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发表时间：
2009-10-01
影响因子：
6.1
通讯作者：
SenGupta, Sioban B.
中科院分区：
文献类型：
--
作者：
Jaroudi, Souraya;Kakourou, Georgia;SenGupta, Sioban B.
早期植入前胚胎依赖于来自卵母细胞的mRNA和蛋白来检测DNA损伤并激活DNA修复、细胞周期停滞或凋亡。在哺乳动物卵母细胞和胚胎中已经检测到一些修复基因的表达，然而，对人类胚泡中DNA修复基因的表达知之甚少。在本研究中，DNA修复基因在人类卵母细胞和囊胚中的表达进行了研究，以确定在这些阶段涉及的途径，并检测胚胎基因组激活前后修复机制的潜在差异。在研究的154个DNA修复基因中，109个在胚泡中检测到，107个在卵母细胞中检测到。在差异表达的DNA修复基因中，有40/55（73%）的基因在囊胚中的表达水平低于卵母细胞（P < 0.05，倍数变化> 3）。尽管由于培养或冻融的限制，大量的修复基因被检测到，这表明所有的DNA修复途径在人类卵母细胞和囊胚中都是潜在的功能性的。与胚泡相比，卵母细胞中大多数修复基因的较高mRNA水平确保了在胚胎基因组激活之前模板的充分可用性。
The early preimplantation embryo relies on mRNA and protein from the oocyte to detect DNA damage and activate DNA repair, cell cycle arrest or apoptosis. Expression of some repair genes has been detected in mammalian oocytes and embryos; however, little is known about DNA repair gene expression in human blastocysts. In this study, DNA repair gene expression was investigated in human oocytes and blastocysts to identify the pathways involved at these stages and detect potential differences in repair mechanisms pre- and post-embryonic genome activation.Triplicate sets of pooled metaphase II oocytes or blastocysts were processed for analysis using the Human Genome Survey Microarrays V2.0 (Applied Biosystems).Of 154 DNA repair genes investigated, 109 were detected in blastocysts and 107 in oocytes. Among differentially expressed DNA repair genes, 40/55 (73%) had lower expression levels in blastocysts compared with oocytes (P < 0.05, fold change > 3).Despite experimental limitations due to culture or freezing and thawing of samples, large numbers of repair genes were detected indicating that all DNA repair pathways are potentially functional in human oocytes and blastocysts. The higher mRNA level for most repair genes in oocytes compared with blastocysts ensures sufficient availability of template until embryonic genome activation.