Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
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DOI：
10.1016/j.biombioe.2020.105697
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发表时间：
2020-10-01
影响因子：
6
通讯作者：
Goldbeck, Rosana
中科院分区：
文献类型：
--
作者：
Barbosa, Fernando Cesar;Kendrick, Emanuele;Goldbeck, Rosana
木质纤维素生物质的酶水解占第二代生物乙醇生产总成本的20-30%，近年来已经做出了许多努力来克服酶的高成本。使用纤维素低聚糖（COS）（纤维素转化为葡萄糖的中间体）可以提供优于单体葡萄糖发酵的优点，例如过程污染物生长的风险较低、发酵时间较短以及高浓度葡萄糖对过程的抑制有限。此外，COS还可用作食品和饲料领域的功能性低聚糖。本研究旨在优化COS生产，以进一步工业应用。据我们所知，这是第一个研究，已使用的实验设计方法来分析内切葡聚糖酶，溶解性多糖单加氧酶（LPMO），纤维二糖脱氢酶（CDH）和不同的添加剂之间的协同作用，在水解预处理甘蔗秸秆生产COS。在优化酶水解后，内切葡聚糖酶CaCel和CcCel 9 m、LPMO TrCe 161 A、CDH NcCDHIIa与乳糖和铜作为添加剂的组合产生60.49 mg COS/g预处理的甘蔗秸秆，比商业酶混合物Cellic（R）Ctec 2和Celluclast（R）1.5 L多1.8-2.7倍。达到的COS/葡萄糖比为298.31，分别比商业酶混合物增加了3314和2294倍。这些结果为COS的生产及其工业应用开辟了新的前景。
Enzymatic hydrolysis of lignocellulosic biomass accounts for 20-30% of the total cost of second-generation bioethanol production and many efforts have been made in recent years to overcome the high cost of enzymes. Using cello-oligosaccharides (COS), intermediates in cellulose conversion to glucose, may provide advantages over monomeric glucose fermentation, such as lower risk of growth of process contaminants, shorter fermentation time and limited process inhibition by high concentrations of glucose. In addition, COS are also useful as functional oligosaccharides in the food and feed sectors. This study aimed to optimize COS production for further industrial applications. To the best of our knowledge, this is the first study that has used a design of experiments approach to analyze the synergism between endoglucanases, lytic polysaccharide monooxygenase (LPMO), cellobiose dehydmgenase (CDH) and different additives during the hydrolysis of a pretreated sugarcane straw for COS production. After optimization of enzymatic hydrolysis, a combination of the endoglucanases CaCel and CcCel9m, the LPMO TrCe161A, the CDH NcCDHIIa, with lactose and copper as additives, produced 60.49 mg of COS per g of pretreated sugarcane straw, 1.8-2.7-fold more than the commercial enzyme cocktails Cellic (R) Ctec2 and Celluclast (R) 1.5 L. The COS/glucose ratio achieved was 298.31, an increase of 3314 and 2294-fold over the commercial enzymatic cocktails, respectively. These results open a new perspective regarding COS production and its industrial application.