LIQUID-CRYSTALLINE, PHAGE-LIKE PACKING OF ENCAPSIDATED DNA IN HERPES-SIMPLEX VIRUS
LIQUID-CRYSTALLINE, PHAGE-LIKE PACKING OF ENCAPSIDATED DNA IN HERPES-SIMPLEX VIRUS
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DOI：
10.1016/0092-8674(91)90324-r
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发表时间：
1991-03-08
期刊：
影响因子：
64.5
通讯作者：
STEVEN, AC
中科院分区：
文献类型：
--
作者：
BOOY, FP;NEWCOMB, WW;STEVEN, AC
HSV-1衣壳内的DNA组织已通过冷冻电子显微镜和图像重建确定。 将完全包装的纯化的C-衣壳与空的A-衣壳进行比较。与A-衣壳不同，C-衣壳显示出精细的条纹和间距约为2.6 nm的点状阵列。 包装的DNA形成均匀致密的球，径向延伸至二十面体（T = 16）衣壳壳的内表面，其结构在A-衣壳和C-衣壳中基本相同。 因此，我们没有发现Schrag等人先前报道的T = 4的内壳存在于C-衣壳中的证据。 封装的HSV-1 DNA与先前在噬菌体T4和λ中观察到的非常相似，因此支持动物病毒（疱疹病毒）的主要家族和细菌病毒的主要家族的各自组装途径之间的密切平行性的想法。
The organization of DNA within the HSV-1 capsid has been determined by cryoelectron microscopy and image reconstruction. Purified C-capsids, which are fully packaged, were compared with A-capsids, which are empty. Unlike A-capsids, C-capsids show fine striations and punctate arrays with a spacing of approximately 2.6 nm. The packaged DNA forms a uniformly dense ball, extending radially as far as the inner surface of the icosahedral (T = 16) capsid shell, whose structure is essentially identical in A-capsids and C-capsids. Thus we find no evidence for the inner T = 4 shell previously reported by Schrag et al. to be present in C-capsids. Encapsidated HSV-1 DNA closely resembles that previously visualized in bacteriophages T4 and lambda, thus supporting the idea of a close parallelism between the respective assembly pathways of a major family of animal viruses (the herpesviruses) and a major family of bacterial viruses.