Analysis of intraspecific variation of Chinese Carthamus tinctorius L. using AFLP markers.
Analysis of intraspecific variation of Chinese Carthamus tinctorius L. using AFLP markers.
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发表时间：
2006
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通讯作者：
Lei Zhang;Bei-Bei Huang-Bei;G. Kai;Meili Guo
中科院分区：
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作者：
Lei Zhang;Bei-Bei Huang-Bei;G. Kai;Meili Guo
目的研究红花的种内变异。为红花种质资源的进一步选育和品质相关基因的筛选奠定基础。方法采用扩增片段长度多态性（AFLP）技术对我国28个红花居群的遗传变异进行分析。采用UPGMA聚类分析方法构建了系统树，并估算了各居群间的遗传距离。结果12对引物组合可以区分所有的群体。居群间的相似系数在0.48 ~ 0.96之间。聚类分析表明，所有品种明显分离成三个主群和一个中间群。结论供试的28个种质资源在种内水平存在有限的遗传多样性，AFLP标记与形态标记的遗传多样性不完全一致，可能是基因型与环境互作的结果。
AIM To investigate the intraspecific variation of Carthamus tinctorius L. (safflower) and establish foundation for further breeding of safflower germplasm resource and screening the quality correlation genes. METHODS Amplified fragment length polymorphism (AFLP) was carried out to analyze genetic variation of 28 safflower populations collected in China. Unweighed pair-group method of with arithmetical averages (UPGMA) cluster analysis was used to construct a dendrogram and to estimate the genetic distances among the populations. RESULTS All populations could be uniquely distinguished using 12 selected primer combinations. Similarity coefficients ranged from 0.48 to 0.96 among the populations. Dendrogram revealed distinct segregation of all the cultivars into three main groups and one midst group. CONCLUSION Limited genetic diversity exists within the tested 28 collections at intra specific level and AFLP-based phyiogeny was not absolutely consistent with that based on morphological characters may be due to the interaction effect between genotype and environment.