Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate.
Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate.
复制标题
修订红色抗生素灵菌红素生物合成的第一步：使用合成硫酯验证新中间体。
DOI：
10.1039/d0cb00173b
复制
发表时间：
2021-04-01
影响因子：
4.1
通讯作者：
Leeper FJ
中科院分区：
文献类型：
--
作者：
Couturier M;Bhalara HD;Monson RE;Salmond GPC;Leeper FJ
红色抗生素灵芝菌素的生物合成途径早在十多年前就被提出，但并不是所有建议的中间体都可以在实验中检测到。在这里，我们证明了最初不包括在途径中的硫代酯是中间体。此外，PIGE酶最初被描述为转氨酶，但我们提出的证据表明，它还催化硫酯中间体还原为其醛底物。对通过一种新的硫酯中间体合成著名的红色素灵芝球蛋白的生物合成途径进行了修订。
A biosynthetic pathway for the red-antibiotic, prodigiosin, was proposed over a decade ago but not all the suggested intermediates could be detected experimentally. Here we show that a thioester that was not originally included in the pathway is an intermediate. In addition, the enzyme PigE was originally described as a transaminase but we present evidence that it also catalyses the reduction of the thioester intermediate to its aldehyde substrate. A revision is proposed to the biosynthetic pathway to the well-known red pigment prodigiosin via a new thioester intermediate.