Analysis of respiratory quinones in soil for characterization of microbiota
Analysis of respiratory quinones in soil for characterization of microbiota
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DOI：
10.1080/00380768.1998.10414461
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发表时间：
1998-09-01
影响因子：
2
通讯作者：
Katayama, A
中科院分区：
文献类型：
--
作者：
Fujie, K;Hu, HY;Katayama, A
建立了土壤中呼吸性醌类化合物的分析方法，以表征土壤微生物区系。呼吸道醌类化合物用氯仿和甲醇的混合物提取，使用Wahling搅拌器或超声仪，并通过硅胶柱柱净化。醌物种测定通过反相高效液相色谱法。用光电二极管阵列检测器测量峰的光谱以检查纯度。三次萃取可回收土壤中90%以上的可萃取醌类化合物。以微生物冻干粉添加到土壤中，回收率大于96%。这一程序产生了更高的代表性结果，如下所示。95%累积频率内的波动分别为13%的醌类提取量和20%的差异，分别。测定了稻田好氧层土壤、安藤土和两种施用化肥和不施用农家肥的高地中醌的含量。土壤中的微生物组成和微生物多样性的醌配置文件显示出明显的差异。结果表明，呼吸醌谱的分析是有用的表征土壤微生物群落结构。
Method for the analysis of respiratory quinones in soil was developed to characterize soil microbiota. The respiratory quinones were extracted with a mixture of chloroform and methanol, using a Wahling blender or a sonicator and cleaned-up by a silicagel column cartridge. The quinone species were determined by reverse-phase high performance liquid chromatography. Spectra of peaks mere measured with a photodiode array detector to examine the purity. More than 90% of extractable quinones in soil were recovered by three extractions. The recovery of quinones, added to soil as freeze-dried powder of microorganisms, was higher than 96%. This procedure led to higher representative results as follows. Fluctuations within 95% of cumulative frequency were 13% for the extracted amount of quinones and 20% for the dissimilarity, respectively. The quinone profiles of four soils mere determined as follows: a soil from the aerobic layer of a paddy field, an ando soil and two yellow upland soils which had received chemical fertilizers with and without farmyard manure. The quinone profiles displayed clear differences in the microbial composition and in the microbial diversity among the soils. It was demonstrated that the analysis of the respiratory quinone profile was useful to characterize the microbial community structure in soil.