The gene MACCHI-BOU 4/ENHANCER OF PINOID encodes a NPH3-like protein and reveals similarities between organogenesis and phototropism at the molecular level
The gene MACCHI-BOU 4/ENHANCER OF PINOID encodes a NPH3-like protein and reveals similarities between organogenesis and phototropism at the molecular level
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DOI：
10.1242/dev.009654
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发表时间：
2007-11-01
期刊：
影响因子：
4.6
通讯作者：
Tasaka, Masao
中科院分区：
文献类型：
--
作者：
Furutani, Masahiko;Kajiwara, Takahito;Tasaka, Masao
植物生长素的细胞间运输是植物器官发生的重要因素。为了研究生长素控制器官发生的分子机制，我们分析了被鉴定为pinoid（pid）增强子的macchi-bou 4（mab 4）突变体。尽管mab 4和pid单突变体显示出相对温和的子叶表型，但pid mab 4双突变体完全缺乏子叶。我们发现MAB 4与已被认为控制子叶原基中PIN 1极性的PIN增强子（ENP）相同。MAB 4/ ENP编码一种新的蛋白质，其属于非向光性下胚轴3（NPH 3）家族，被认为在向光性中起信号转导器的作用并控制生长素的侧向移位。MAB 4/ ENP mRNA在胚胎的原胚层细胞层和器官起始部位的分生组织L1层中检测到。在mab 4胚胎中，PIN 1：GFP的丰度在原皮细胞层的质膜处严重降低。此外，亚细胞定位分析表明，MAB 4/ ENP驻留在一个亚群的内体以及对身份不明的细胞内区室。这些结果表明MAB 4/ ENP参与了器官发生过程中生长素的极性运输。
Intercellular transport of the phytohormone auxin is a significant factor for plant organogenesis. To investigate molecular mechanisms by which auxin controls organogenesis, we analyzed the macchi- bou 4 ( mab4) mutant identified as an enhancer of pinoid ( pid). Although mab4 and pid single mutants displayed relatively mild cotyledon phenotypes, pid mab4 double mutants completely lacked cotyledons. We found that MAB4 was identical to ENHANCER OF PINOID ( ENP), which has been suggested to control PIN1 polarity in cotyledon primordia. MAB4/ ENP encodes a novel protein, which belongs to the NON- PHOTOTROPIC HYPOCOTYL 3 ( NPH3) family thought to function as a signal transducer in phototropism and control lateral translocation of auxin. MAB4/ ENP mRNA was detected in the protodermal cell layer of the embryo and the meristem L1 layer at the site of organ initiation. In the mab4 embryo, the abundance of PIN1: GFP was severely decreased at the plasma membrane in the protodermal cell layer. In addition, subcellular localization analyses indicated that MAB4/ ENP resides on a subpopulation of endosomes as well as on unidentified intracellular compartments. These results indicate that MAB4/ ENP is involved in polar auxin transport in organogenesis.