X-ray crystallographic characterization and phasing of a fucose-specific lectin from Aleuria aurantia
X-ray crystallographic characterization and phasing of a fucose-specific lectin from Aleuria aurantia
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DOI：
10.1107/s0907444902022175
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发表时间：
2003-02-01
影响因子：
2.2
通讯作者：
Miki, K
中科院分区：
文献类型：
--
作者：
Fujihashi, M;Peapus, DH;Miki, K
从Aleuria aurantia的岩藻糖特异性凝集素结晶在其天然形式，也与氯化汞共结晶。采用坐滴气相扩散法，以硫酸铵为沉淀剂进行结晶。无汞天然晶体和汞共晶都属于六方晶系空间群P6（5）22。无Hg形式的晶胞参数为a = 84.0，c = 250.1埃，Hg共晶的晶胞参数为a = 83.9，c = 254.3埃。这两种形式的晶体都能将X射线的分辨率提高到2.3埃，适用于高分辨率的晶体结构测定。初始阶段成功地进行了MAD方法使用汞共晶和电子密度得到足够好的模型建设。
A fucose-specific lectin from Aleuria aurantia was crystallized in its native form and was also cocrystallized with HgCl2. Crystallization was performed using the sitting-drop vapour-diffusion method with ammonium sulfate as a precipitant. Both the Hg-free native crystals and the Hg cocrystals belong to the hexagonal space group P6(5)22. The unit-cell parameters for the Hg-free form are a = 84.0, c = 250.1 Angstrom and for the Hg cocrystals are a = 83.9, c = 254.3 Angstrom. Both forms of the crystals diffract X-rays to 2.3 Angstrom resolution and are suitable for high-resolution crystal structure determination. Initial phasing was successfully performed by the MAD method using the Hg cocrystals and the electron density obtained was good enough for model building.