Anti-fatigue activity of purified anthocyanins prepared from purple passion fruit (P. edulis Sim) epicarp in mice
Anti-fatigue activity of purified anthocyanins prepared from purple passion fruit (P. edulis Sim) epicarp in mice
复制标题
紫百香果（P. edulis Sim）外果皮制备的纯化花青素对小鼠的抗疲劳活性
DOI：
10.1016/j.jff.2019.103725
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Xiong, Jianhua
中科院分区：
文献类型：
--
作者：
Hu, Min;Du, Juan;Xiong, Jianhua
由于花色苷在人体健康和食用着色剂中具有积极的生物活性，对花色苷的需求正在稳步增长。本研究对纯化的紫百香果外果皮花色苷(PFEA)的性质及其抗疲劳作用及其机制进行了研究。结果表明，分离鉴定出3种主要花色苷：花青素-3-O-葡萄糖苷、花青素-3-O-芦丁糖苷和牡丹素-3-O-葡萄糖苷，总含量为710.40 mg/g。PFEA能显著延长小鼠负重强迫游泳时间，降低乳酸脱氢酶(LDH)、血乳酸(BLA)和血尿素氮(BUN)水平，升高肝糖原(LG)含量。同时，PFEA可改善氧化应激生物标志物(MDA)、抗氧化酶(SOD)和炎性细胞因子(TNF-α、IL-1β、IL-6)的波动。此外，PFEA还上调了骨骼肌中PGC-1α和PPARα的mRNA表达。综上所述，这些结果表明补充PFEA可以缓解疲劳效应。
Demands for anthocyanins are steadily growing due to their positive bioactivities in human health and food coloration. This study was performed to evaluate the characteristics of purified purple passion fruit epicarp anthocyanins (PFEA) as well as its anti-fatigue activity and underlying mechanisms in mice. The result showed that three major anthocyanins including cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-glucoside were identified, and the total content of these anthocyanins was 710.40 mg/g in PFEA. PFEA significantly prolonged the weight-loaded forced swimming time, decreased lactate dehydrogenase (LDH), the blood lactate (BLA) and the blood urea nitrogen (BUN) levels, and increased the liver glycogen (LG) content. Meanwhile, PFEA could ameliorate fluctuates in oxidative stress biomarkers (MDA), antioxidant enzymes (SOD) and inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6). Moreover, the mRNA expression of PGC-1 alpha and PPAR alpha in skeletal muscles were also up-regulated by PFEA treatment. In conclusion, these results showed that supplementing PFEA could relieve fatigue effects.