Rubiscolin-6, a δ opioid peptide derived from spinach Rubisco, has anxiolytic effect via activating σ1, and dopamine D1 receptors
Rubiscolin-6, a δ opioid peptide derived from spinach Rubisco, has anxiolytic effect via activating σ1, and dopamine D1 receptors
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DOI：
10.1016/j.peptides.2007.07.024
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发表时间：
2007-10-01
期刊：
影响因子：
3
通讯作者：
Yoshikawa, Masaaki
中科院分区：
文献类型：
--
作者：
Hirata, Hajime;Sonoda, Soushi;Yoshikawa, Masaaki
Rubiscolin-6（Tyr-Pro-Leu-Asp-Leu-Phe）是菠菜D-核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco）大亚基衍生的阿片肽。我们以前报道，rubiscolin-6有镇痛作用，并刺激记忆巩固。在这里，我们表明腹腔内（i. p.）或口服给药的rubiscolin-6在小鼠高架十字迷宫试验中分别以10 mg/kg或100 mg/kg的剂量具有抗焦虑作用。在i. p.（10 mg/kg）和口服（100 mg/kg）给药后，rubscolin-6的抗焦虑作用被8阿片受体拮抗剂纳曲吲哚（1 mg/kg，s.c.）提示rubiscolin-6的抗焦虑活性是通过6阿片受体介导的。rubiscolin-6（10mg/kg，i.p.）也被多巴胺D1拮抗剂SCH 23390（30 μ g/kg，i. p.），但不被多巴胺D-2拮抗剂雷氯必利（15 μ g/kg，i. p.）rubiscolin-6（10mg/kg，i.p.）受体拮抗剂BMY 14802（0.5mg/kg，i. p.）或BD 1047（10 mg/kg，i.p.）。综上所述，rubiscolin-6的抗焦虑作用由δ阿片受体下游的σ（1）和多巴胺D-1受体介导。(C)2007年爱思唯尔公司All rights reserved.
Rubiscolin-6 (Tyr-Pro-Leu-Asp-Leu-Phe) is a opioid peptide derived from the large subunit of spinach D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). We previously reported that rubiscolin-6 had an analgesic effect and stimulated memory consolidation. Here we show that intraperitoneally (i.p.) or orally administered rubiscolin-6 has an anxiolytic effect at a dose of 10 mg/kg or 100 mg/kg, respectively, in the elevated plus-maze test in mice. The anxiolytic effects of rubscolin-6 after i.p. (10 mg/kg) and oral (100 mg/kg) administration were blocked by a 8 opioid receptor antagonist, naltrindole (1 mg/kg, s.c.), suggesting that the anxiolytic activity of rubiscolin-6 is mediated by 6 opioid receptor. The anxiolytic effect of rubiscolin-6 (10 mg/kg, i.p.) was also blocked by a dopamine D, antagonist, SCH23390 (30 mu g/kg, i.p.), but not by a dopamine D-2 antagonist, raclopride (15 mu g/kg, i.p.). The anxiolytic effect of rubiscolin-6 (10 mg/kg, i.p.) was blocked by (T, receptor antagonist, BMY14802 (0.5 mg/kg, i.p.) or BD1047 (10 mg/kg, i.p.). Taken together, the anxiolytic effect of rubiscolin-6 is mediated by sigma(1) and dopamine D-1 receptors downstream of 8 opioid receptor. (C) 2007 Elsevier Inc. All rights reserved.