Microalgal swimming signatures and neutral lipids production across growth phases
Microalgal swimming signatures and neutral lipids production across growth phases
复制标题
微藻游动特征和整个生长阶段的中性脂质产生
DOI：
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发表时间：
2020
影响因子：
3.8
通讯作者：
M. Hondzo
中科院分区：
文献类型：
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作者：
Jiaqi You;K. Mallery;D. Mashek;M. Sanders;Jiarong Hong;M. Hondzo
微藻已被证明是一种潜在的食品、生物燃料和制药产品的生物资源。在相应环境条件下的生长阶段，微藻积累了不同数量的各种代谢物。我们量化了中性脂的积累，并分析了运动绿藻杜氏藻（Dunaliella primolecta）在不同营养浓度下的滞后-指数-平稳生长周期中的游泳特征（速度和轨迹）。我们发现了微藻在生长阶段中性脂含量和游泳特征的显著变化。最大游动速度与最大中性脂含量出现的时间一致，这两个最大值都出现在营养胁迫下的固定生长期。此外，当观察到细胞内中性脂含量最高时，游泳轨迹表明在平稳生长阶段游泳模式发生了统计学上显著的变化。我们的研究结果提供了利用微藻游泳特征作为培养条件和微藻收获时间的可能指标，以最大限度地提高生物燃料生产的脂质产量的潜力。这一发现也可用于探索利用其他微藻代谢物以低能量成本生产食物和抗生素。
Microalgae have been shown as a potential bioresource for food, biofuel, and pharmaceutical products. During the growth phases with corresponding environmental conditions, microalgae accumulate different amounts of various metabolites. We quantified the neutral lipids accumulation and analyzed the swimming signatures (speed and trajectories) of the motile green alga, Dunaliella primolecta, during the lag–exponential–stationary growth cycle at different nutrient concentrations. We discovered significant changes in the neutral lipid content and swimming signatures of microalgae across growth phases. The timing of the maximum swimming speed coincided with the maximum neutral lipid content and both maxima occurred under nutrient stress at the stationary growth phase. Furthermore, the swimming trajectories suggested statistically significant changes in swimming modes at the stationary growth phase when the maximum intracellular neutral lipid content was observed. Our results provide the potential exploitation of microalgal swimming signatures as possible indicators of the cultivation conditions and the timing of microalgal harvest to maximize the lipid yield for biofuel production. The findings can also be implemented to explore the production of food and antibiotics from other microalgal metabolites with low energy costs.