Temperature-Sensitive Elastin-Mimetic Dendrimers: Effect of Peptide Length and Dendrimer Generation to Temperature Sensitivity
Temperature-Sensitive Elastin-Mimetic Dendrimers: Effect of Peptide Length and Dendrimer Generation to Temperature Sensitivity
复制标题
DOI：
10.1002/bip.22425
复制
发表时间：
2014-06-01
期刊：
影响因子：
2.9
通讯作者：
Tanaka, Naoki
中科院分区：
文献类型：
--
作者：
Kojima, Chie;Irie, Kotaro;Tanaka, Naoki
树枝状聚合物是具有独特结构的合成大分子，是肽的潜在支架。弹性蛋白是细胞外基质的主要成分之一，是一种温度敏感的生物大分子。此前，Val-Pro-Gly-Val-Gly 肽已与树枝状聚合物缀合，用于设计弹性蛋白模拟树枝状聚合物。在本研究中，合成了使用不同长度肽和不同代树枝状聚合物的各种弹性蛋白模拟树枝状聚合物以控制温度依赖性。模拟弹性蛋白树枝状大分子通过加热形成类似于弹性蛋白样肽的-转角结构。弹性蛋白模拟树枝状聚合物表现出反相变，很大程度上取决于肽长度，并且稍微取决于树枝状聚合物的生成。弹性蛋白模拟树枝状聚合物在相变后形成聚集体。在具有长肽的弹性蛋白模拟树枝状聚合物中观察到吸热峰，但在具有短肽的弹性蛋白模拟树枝状聚合物中观察到吸热峰。肽长度和树枝状聚合物的生成是调节弹性蛋白模拟树枝状聚合物的温度依赖性的重要因素。 (c) 2013 Wiley periodicals, Inc. 生物聚合物 101: 603-612, 2014。
Dendrimers are synthetic macromolecules with unique structure, which are a potential scaffold for peptides. Elastin is one of the main components of extracellular matrix and a temperature-sensitive biomacromolecule. Previously, Val-Pro-Gly-Val-Gly peptides have been conjugated to a dendrimer for designing an elastin-mimetic dendrimer. In this study, various elastin-mimetic dendrimers using different length peptides and different dendrimer generations were synthesized to control the temperature dependency. The elastin-mimetic dendrimers formed -turn structure by heating, which was similar to the elastin-like peptides. The elastin-mimetic dendrimers exhibited an inverse phase transition, largely depending on the peptide length and slightly depending on the dendrimer generation. The elastin-mimetic dendrimers formed aggregates after the phase transition. The endothermal peak was observed in elastin-mimetic dendrimers with long peptides, but not with short ones. The peptide length and the dendrimer generation are important factors to tune the temperature dependency on the elastin-mimetic dendrimer. (c) 2013 Wiley Periodicals, Inc. Biopolymers 101: 603-612, 2014.