Purification of Synthetic Oligonucleotides via Catching by Polymerization
Purification of Synthetic Oligonucleotides via Catching by Polymerization
复制标题
通过聚合捕获纯化合成寡核苷酸
DOI：
10.1002/0471142700.nc1014s49
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发表时间：
2012
影响因子：
--
通讯作者：
Yinan Yuan
中科院分区：
文献类型：
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作者：
Shiyue Fang;Suntara Fueangfung;Yinan Yuan
本单元描述了使用聚合捕获方法纯化合成的寡脱氧核糖核苷酸（ODN）。在粗ODN中，主要杂质是在每个合成循环的偶联步骤中产生的失效序列。由于它们的物理性质与全长序列相似，因此难以去除。本单元描述了两种非色谱方法来解决该问题。在第一种方法中，在自动化合成过程中，失败序列用甲基丙烯酰胺基团标记，该基团是可聚合的并且可以参与丙烯酰胺自由基聚合反应;全长序列没有标记。合成后，使粗混合物进行聚合。将失效序列并入不溶性聚合物中;用水提取全长序列。在第二种方法中，全长序列通过可切割接头用甲基丙烯酰胺基团标记;失败序列不标记。合成后，将全长序列掺入聚合物中;用水冲洗掉失效序列。从聚合物上切割纯全长序列。这两种方法是互补的。Curr.方案核酸化学49：10.14.1 - 10.14.21。© 2012年由John Wiley & Sons，Inc.
This unit describes the purification of synthetic oligodeoxyribonucleotides (ODN) using a catching‐by‐polymerization approach. In a crude ODN, the major impurity is the failure sequences generated in the coupling step of each synthetic cycle. They are difficult to remove due to the similarity of their physical properties to the full‐length sequences. Two non‐chromatographic methods are described in the unit to solve the problem. In the first one, during automated synthesis, the failure sequences are tagged with a methacrylamide group, which is polymerizable and can participate in acrylamide radical polymerization reactions; the full‐length sequences are not tagged. After synthesis, the crude mixture is subjected to polymerization. The failure sequences are incorporated into an insoluble polymer; the full‐length sequences are extracted with water. In the second method, the full‐length sequences are tagged with a methacrylamide group via a cleavable linker; the failure sequences are not tagged. After synthesis, the full‐length sequences are incorporated into a polymer; the failure sequences are washed away with water. Pure full‐length sequences are cleaved from the polymer. The two methods are complementary. Curr. Protoc. Nucleic Acid Chem. 49:10.14.1‐10.14.21. © 2012 by John Wiley & Sons, Inc.
小麦胚芽凝集素与胶体金单层上积累的携带 N-乙酰氨基葡萄糖的端粒刷的相互作用
DOI：
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发表时间：
2008
期刊：
Biointerfaces 61
影响因子：
--
作者：
Kitano;H. ; Nakada;H. ; Mizukami;K.;Colloids Surfaces B
通讯作者：
Colloids Surfaces B