Human amniotic fluid stem cells labeled with up-conversion nanoparticles for imaging-monitored repairing of acute lung injury
Human amniotic fluid stem cells labeled with up-conversion nanoparticles for imaging-monitored repairing of acute lung injury
复制标题
用上转换纳米颗粒标记的人羊水干细胞用于成像监测修复急性肺损伤
DOI：
10.1016/j.biomaterials.2016.05.034
复制
发表时间：
2016
期刊：
影响因子：
14
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Xu YY;Xiang J;Zhao H;Liang HS;Huang J;Li Y;Pan J;Zhou HT;Zhang XG;Wang J H;Liu Z;Wang J
人羊水干细胞（hAFS）在细胞治疗和再生医学中产生了很大的兴奋。在这里，我们研究了双聚合物包被的UCNP-PEG-PEI纳米颗粒标记的hAFS细胞在急性肺损伤（ALI）小鼠模型中的作用。我们使用高灵敏度的体内上转换发光（UCL）成像观察到hAFS细胞迁移到肺。我们证明，hAFS细胞保持活力，并保留其分化能力，即使在UCNP-PEG-PEI标记。与小鼠骨髓间充质干细胞（mBMSCs）相比，hAFS细胞对急性肺损伤的肺组织修复具有显著的促进作用，包括恢复肺泡-毛细血管膜的完整性，减少白细胞和中性粒细胞的跨上皮迁移，下调促炎细胞因子和趋化因子的表达。我们的工作突出了成像引导的hAFS细胞为基础的治疗在ALI中的一个有前途的作用。
Human amniotic fluid stem (hAFS) cells have generated a great deal of excitement in cell-based therapies and regenerative medicine. Here, we examined the effect of hAFS cells labeled with dual-polymer-coated UCNP-PEG-PEI nanoparticles in a murine model of acute lung injury (ALI). We observed hAFS cells migration to the lung using highly sensitive in vivo upconversion luminescence (UCL) imaging. We demonstrated that hAFS cells remained viable and retained their ability to differentiate even after UCNP-PEG-PEI labeling. More importantly, hAFS cells displayed remarkable positive effects on ALI-damaged lung tissue repair compared with mouse bone marrow mesenchymal stem cells (mBMSCs), which include recovery of the integrity of alveolar-capillary membrane, attenuation of transepithelial leukocyte and neutrophil migration, and down-regulation of proinflammatory cytokine and chemokine expression. Our work highlights a promising role for imaging-guided hAFS cell-based therapy in ALI.