Open reading frames of turnip crinkle virus involved in satellite symptom expression and incompatibility with Arabidopsis thaliana ecotype Dijon.
Open reading frames of turnip crinkle virus involved in satellite symptom expression and incompatibility with Arabidopsis thaliana ecotype Dijon.
复制标题
萝卜皱纹病毒的开放阅读框涉及卫星症状表达以及与拟南芥生态型第戎的不相容性。
DOI：
10.1094/mpmi-8-0979
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
A. Simon
中科院分区：
文献类型：
--
作者：
Jong;Q. Kong;C. Song;C. D. Carpenter;A. Simon
CarmoVirus是一种单链、单组分的RNA病毒，包括芜菁皱纹病毒(TCV)和最近发现的豆蔻绿斑点病毒(CCFV)。分别为CCFV的TCV-M分离物和蓝湖分离物构建了具有生物活性的全长cDNA。利用构建在TCV分离株之间的嵌合病毒，当与毒力卫星RNA共同接种时，产生轻微或严重症状的TCV分离株之间的嵌合病毒，聚合酶开放阅读框中1,144位的Glu残基被鉴定参与卫星介导的症状表达。为了分析参与抗性的病毒决定因素，在不侵染拟南芥生态型Dijon(Di-0)的TCV和能侵染Di-0的CCFV之间产生了具有精确互换开放读框的嵌合病毒，而带有CCFV外壳蛋白的TCV能够系统地侵染Di-0，尽管全株杂交表明杂交病毒的传播速度比两种亲本病毒中的任何一种都慢。这些结果表明，这两个亲本病毒。这些结果表明，外壳蛋白是Di-0对TCV抗性的一个重要的病毒决定因素。
Carmoviruses are single-stranded, single component RNA viruses that include turnip crinkle virus (TCV) and the recently discovered cardamine chlorotic fleck virus (CCFV). Full-length, biologically active cDNAs were constructed for the TCV-M isolate and the Blue Lake isolate of CCFV. Using chimeric viruses constructed between isolates of TCV that produce mild or severe symptoms when coinoculated with a virulent satellite RNA, a Glu residue at position 1,144 in the polymerase open reading frame was identified as being involved in satellite-mediated symptom expression. To analyze viral determinants involved in resistance, chimeric viruses with precisely exchanged open reading frames were produced between TCV, which does not infect the Arabidopsis thaliana ecotype Dijon (Di-0), and CCFV, which can infect Di-0, TCV with the coat protein of CCFV was able to systemically infect Di-0 although whole plant hybridizations revealed that the hybrid virus spread more slowly than either of the two parental viruses. These results indicate that the two parental viruses. These results indicate that the coat protein is an important viral determinant in the resistance of Di-0 to TCV.