Heterogeneous expression pattern of pro- and anti-apoptotic factors in myeloid progenitor cells of patients with severe congenital neutropenia treated with granulocyte colony-stimulating factor
Heterogeneous expression pattern of pro- and anti-apoptotic factors in myeloid progenitor cells of patients with severe congenital neutropenia treated with granulocyte colony-stimulating factor
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DOI：
10.1111/j.1365-2141.2005.05428.x
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发表时间：
2005-04-01
影响因子：
6.5
通讯作者：
Welte, K
中科院分区：
文献类型：
--
作者：
Cario, G;Skokowa, J;Welte, K
严重先天性中性粒细胞减少症（CN）患者的髓系祖细胞凋亡加速。粒细胞集落刺激因子（G-CSF）增加大多数CN患者的中性粒细胞数量。通过细胞凋亡率、抗凋亡因子和促凋亡因子的表达分析G-CSF对CN细胞凋亡的影响。G-CSF治疗组细胞凋亡率增高，bcl-2和bcl-xL表达降低，bfl-1/A1和mcl-1表达增高。G-CSF给药后，Caspase 9在患者和对照组中高度表达。因此，G-CSF作用于细胞凋亡调节，但必须假设导致中性粒细胞数量增加的其他机制。
Apoptosis is accelerated in the myeloid progenitor cells of patients with severe congenital neutropenia (CN). Granulocyte colony-stimulating factor (G-CSF) increases neutrophil numbers in most CN patients. The effect of G-CSF on apoptosis in CN was analysed by apoptosis rate and expression of anti- and pro-apoptotic factors. G-CSF-treated patients showed higher apoptosis frequency, lower expression of bcl-2 and bcl-xL, but higher expression of bfl-1/A1 and mcl-1. Caspase 9 was highly expressed in patients and controls after G-CSF administration. Thus, G-CSF acts on apoptosis regulation, but additional mechanisms leading to the increase of neutrophil numbers must be assumed.