Selective stimulation of translational expression by Alu RNA
Selective stimulation of translational expression by Alu RNA
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DOI：
10.1093/nar/gkf419
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发表时间：
2002-07-15
影响因子：
14.9
通讯作者：
Schmid, CW
中科院分区：
文献类型：
--
作者：
Rubin, CM;Kimura, RH;Schmid, CW
在共瞬时转染试验中，人Alu和腺病毒VA1 rna分别刺激报告基因的翻译表达，而不影响整体蛋白质合成速率或报告mRNA的丰度。这种选择性的、转录后的表达刺激，在人类和小鼠细胞系以及三个报告者中观察到，是通过一种不依赖于pkr的机制起作用的。在这个实验中，Alu和VA1 rna的活性是短暂的，导致新合成的报告mrna翻译表达的滞后时间减少。延迟时间的减少说明了对报告基因表达影响的相对选择性，并提示Alu和VA1 RNA在细胞应激恢复和病毒感染中的新作用。缺失分析表明，在二聚体Alu一致序列的右侧单体内的特定区域是活性所必需的。大量的左Alu单体转录本是不活跃的，而右Alu单体是完全活跃的，尽管它的转录本很少。小鼠B1和B2 SINE rna刺激小鼠细胞中的报告基因表达，表明这种活性是真核生物SINE的普遍特性。
Human Alu and adenovirus VA1 RNAs each stimulate the translational expression of reporter genes in co-transient transfection assays without affecting either the rate of global protein synthesis or the abundance of the reporter mRNA. This selective, post-transcriptional stimulation of expression, which is observed in human and mouse cell lines and for three reporters, acts through a PKR-independent mechanism. The activity of Alu and VA1 RNAs in this assay is transient, causing a reduction in the lag time for the translational expression of the newly synthesized reporter mRNAs. The reduction in this lag time accounts for the relative selectivity of the effect upon the expression of the reporter and suggests novel roles for Alu and VA1 RNA in cell stress recovery and viral infection. Deletion analysis demonstrates that a specific region residing within the right monomer of the dimeric Alu consensus sequence is necessary for activity. Highly abundant left Alu monomer transcripts are inactive but the right Alu monomer is fully active, although its transcripts are scarce. Mouse B1 and B2 SINE RNAs stimulate reporter gene expression in mouse cells, suggesting that this activity is a general property of eucaryotic SINEs.