Characterization of molecular transport in poly(dimethylsiloxane) microchannels for electrophoresis fabricated with synchrotron radiation-lithography and UV-photolithography
Characterization of molecular transport in poly(dimethylsiloxane) microchannels for electrophoresis fabricated with synchrotron radiation-lithography and UV-photolithography
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DOI：
10.1039/b315674e
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发表时间：
2004-01-01
期刊：
影响因子：
6.1
通讯作者：
Terabe, S
中科院分区：
文献类型：
--
作者：
Liu, BF;Ozaki, M;Terabe, S
本文研究了用紫外光（UV）光刻和同步辐射（SR）光刻制备的聚二甲基硅氧烷微通道中的分子输运，并通过评价通道内的分子分散，对微芯片毛细管电泳进行了表征和比较。使用荧光标记磺酰罗丹明B作为探测分子。结果表明，SR光刻法制备的微通道在分子输运性能方面优于UV光刻法制备的微通道，且具有上级性能。通过扫描电子显微镜对表面状况的深入了解表明，这与表面粗糙度的差异有关。电泳图中的色谱保留进一步支持了这一结论，这取决于通道表面的相比例。在这项工作中所揭示的PDMS微通道的结果与文献中所发现的玻璃微通道的现象非常一致。
In the present paper, a study was undertaken of molecular transport in ploy(dimethylsiloxane) microchannels that were fabricated by ultraviolet (UV)-photolithography and synchrotron radiation (SR)-lithography characterized and compared for microchip capillary electrophoresis by evaluating in-channel molecular dispersion. A fluorescent tag, sulforhodamine B was used as the probing molecule. It was found that microchannels made by SR-lithography fabrication were superior to those made by UV-photolithography fabrication in terms of molecular transport performance. A deep insight into surface conditions characterized by scanning electron microscopy suggested it was related to the difference in surface roughness. Chromatographic retention in electropherograms further supported such a conclusion, which depended on the phase ratio of the channel surface. The results revealed for PDMS microchannels in this work were in good agreement with the phenomenon found for glass microchannels in the literature.