Transcriptomic profiling of tissue environments critical for post-embryonic patterning and morphogenesis of zebrafish skin.
Transcriptomic profiling of tissue environments critical for post-embryonic patterning and morphogenesis of zebrafish skin.
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DOI：
10.7554/elife.86670
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发表时间：
2023-09-11
期刊：
影响因子：
7.7
通讯作者：
Parichy DM
中科院分区：
文献类型：
--
作者：
Aman AJ;Saunders LM;Carr AA;Srivatasan S;Eberhard C;Carrington B;Watkins-Chow D;Pavan WJ;Trapnell C;Parichy DM
色素图案和皮肤附属物是脊椎动物皮肤的显著特征。斑马鱼在胚胎发育后的皮肤上，有规律的色素条纹和一系列钙化的鳞片同时形成。理解调控条纹图案和鳞片形态发生的机制可能会导致发现控制动物形态发展的基本机制。为了了解控制皮肤模式和形态发生的细胞类型和信号相互作用，我们生成并分析了野生型鱼以及遗传或转基因诱导的鳞片或色素沉着缺陷的鱼的皮肤单细胞转录组。这些数据揭示了先前未被描述的表皮细胞群，它们表达编码釉质基质蛋白的转录本，表明激素控制上皮-间质信号传导，阐明了控制鳞状乳头发育的信号网络，并确定了真皮在支持色素细胞发育中的关键作用。此外，这些代表生物医学相关皮肤表型的综合单细胞转录组数据应该为加速发现控制皮肤发育和体内平衡的机制提供有用的资源。
Pigment patterns and skin appendages are prominent features of vertebrate skin. In zebrafish, regularly patterned pigment stripes and an array of calcified scales form simultaneously in the skin during post-embryonic development. Understanding the mechanisms that regulate stripe patterning and scale morphogenesis may lead to the discovery of fundamental mechanisms that govern the development of animal form. To learn about cell types and signaling interactions that govern skin patterning and morphogenesis, we generated and analyzed single-cell transcriptomes of skin from wild-type fish as well as fish having genetic or transgenically induced defects in squamation or pigmentation. These data reveal a previously undescribed population of epidermal cells that express transcripts encoding enamel matrix proteins, suggest hormonal control of epithelial–mesenchymal signaling, clarify the signaling network that governs scale papillae development, and identify a critical role for the hypodermis in supporting pigment cell development. Additionally, these comprehensive single-cell transcriptomic data representing skin phenotypes of biomedical relevance should provide a useful resource for accelerating the discovery of mechanisms that govern skin development and homeostasis.