Improved Drought Tolerance by AMF Inoculation in Maize (Zea mays) Involves Physiological and Biochemical Implications
Improved Drought Tolerance by AMF Inoculation in Maize (Zea mays) Involves Physiological and Biochemical Implications
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DOI：
10.3390/plants8120579
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发表时间：
2019-12-01
期刊：
影响因子：
4.5
通讯作者：
Zhang, Lixin
中科院分区：
文献类型：
--
作者：
Begum, Naheeda;Ahanger, Muhammad Abass;Zhang, Lixin
研究了丛枝菌根真菌（AMF，Glomus versiforme）对干旱胁迫下玉米生长和生理生化特性的改善作用。研究了将玉米植物暴露于两种干旱状态，即，中度干旱（MD）和重度干旱（SD），接种和不接种AMF。干旱在这两个水平降低植物高度，叶绿素和类胡萝卜素含量，从而阻碍光合作用。此外，干旱胁迫增强了有毒活性氧（ROS）的产生，包括H2O2，导致膜损伤反映为增加电解质渗漏和脂质过氧化。这种负面影响在SD条件下比MD和对照更加明显，然而，AMF接种显著改善了干旱诱导的氧化损伤的有害影响。在对照条件下，接种AMF通过显着提高叶绿素含量、矿物质吸收和同化作用来促进生长和光合作用。AMF接种增加了相容性溶质的含量，如脯氨酸，糖和游离氨基酸，有助于维持相对含水量。AMF接种植物的抗氧化系统的上调是明显的，从而介导快速缓解干旱的氧化作用，通过消除活性氧。此外，AMF介导的抗氧化系统的上调有助于维持氧化还原稳态，从而保护主要代谢途径，包括光合作用，如本研究所观察到的。MD和SD条件下，由于AMF接种总酚增加。本研究认为G.在玉米中接种versiforme抵抗干旱胁迫。
The role of arbuscular mycorrhizal fungus (AMF, Glomus versiforme) in amelioration of drought-induced effects on growth and physio-biochemical attributes in maize (Zea mays L.) was studied. Maize plants were exposed to two drought regimes, i.e., moderate drought (MD) and severe drought (SD), with and without AMF inoculation. Drought at both levels reduced plant height, and chlorophyll and carotenoid content, thereby impeding photosynthesis. In addition, drought stress enhanced the generation of toxic reactive oxygen species (ROS), including H2O2, resulting in membrane damage reflected as increased electrolyte leakage and lipid peroxidation. Such negative effects were much more apparent under SD conditions that those of MD and the control, however, AMF inoculation significantly ameliorated the deleterious effects of drought-induced oxidative damage. Under control conditions, inoculation of AMF increased growth and photosynthesis by significantly improving chlorophyll content, mineral uptake and assimilation. AMF inoculation increased the content of compatible solutes, such as proline, sugars and free amino acids, assisting in maintaining the relative water content. Up-regulation of the antioxidant system was obvious in AMF-inoculated plants, thereby mediating quick alleviation of oxidative effects of drought through elimination of ROS. In addition, AMF mediated up-regulation of the antioxidant system contributed to maintenance of redox homeostasis, leading to protection of major metabolic pathways, including photosynthesis, as observed in the present study. Total phenols increased due to AMF inoculation under both MD and SD conditions. The present study advocates the beneficial role of G. versiforme inoculation in maize against drought stress.