Estimation of Organic Carbon Content of the Cyanobacterium Synechococcus sp. by Soft X-ray Microscopy
Estimation of Organic Carbon Content of the Cyanobacterium Synechococcus sp. by Soft X-ray Microscopy
复制标题
DOI：
10.1080/01490451.2015.1010753
复制
发表时间：
2015-04
影响因子：
2.3
通讯作者：
Hisato Ikegaya;Shiro Suzuki;S. Ichise;Seiko Furuta;S. Wakabayashi;T. Ohigashi;Daiya Bamba;H. Namba;H. Kihara;N. Kishimoto;K. Takemoto
中科院分区：
文献类型：
--
作者：
Hisato Ikegaya;Shiro Suzuki;S. Ichise;Seiko Furuta;S. Wakabayashi;T. Ohigashi;Daiya Bamba;H. Namba;H. Kihara;N. Kishimoto;K. Takemoto
建立了一种利用软x射线显微镜（XM）准确测定浮游植物有机碳（OC）含量的方法，并将其应用于细胞外多糖（EPS）覆盖的浮游植物聚藻球菌（Synechococcus sp.）。根据x射线吸收系数和XM图像的灰度值，发现聚藻球菌覆盖eps细胞的OC含量（0.39 ~ 0.47 pg/细胞）是未覆盖eps细胞（0.20 pg/细胞）的2.0 ~ 2.4倍。这些结果表明，软XM可以作为一种有用的工具来评估浮游植物和EPS的OC含量，而无需预处理步骤。
A method for the accurate evaluation of the organic carbon (OC) content of phytoplankton by soft X-ray microscopy (XM) was developed and applied to the picophytoplankton Synechococcus sp., whose cells are covered by extracellular polysaccharides (EPS). Based on the X-ray absorption coefficients and gray levels of the XM images, the OC content of EPS-covered cells of Synechococcus sp. (0.39–0.47 pg/cell) was found to be 2.0–2.4 times larger than that of EPS-removed cells (0.20 pg/cell). These findings suggest that soft XM could be a useful tool for evaluating the OC content of picophytoplankton and EPS without pretreatment steps.