Significance and Expression of Atypical Protein Kinase C-ι in Human Hepatocellular Carcinoma
Significance and Expression of Atypical Protein Kinase C-ι in Human Hepatocellular Carcinoma
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DOI：
10.1016/j.jss.2008.05.036
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发表时间：
2009-06-01
影响因子：
2.2
通讯作者：
Zou, Sheng-Quan
中科院分区：
文献类型：
--
作者：
Wang, Jian-Ming;Li, Qiang;Zou, Sheng-Quan
背景和目的。既往研究表明，非典型蛋白激酶C （aPKC-iota）的异常表达在恶性肿瘤的发生发展中起着至关重要的作用。本研究分析了aPKC-iota与肝癌临床病理及Cyclin E的相关性，探讨了肝癌侵袭转移的分子机制。材料和方法。应用逆转录聚合酶链反应检测7例正常肝组织和43例肝癌及癌旁组织中aPKC-iota基因的表达。免疫组织化学和Western blot检测aPKC-iota和Cyclin E蛋白的表达。最后，我们分析了aPKC-iota与临床病理特征及肝癌侵袭的相关性。aPKC-iota基因在肝癌中的表达值（0.844 +/- 0.315）明显高于癌旁组织的表达值（0.530 +/- 0.217）和正常组织的表达值（0.372 +/- 0.130）（P = 0.009）。aPKC-iota蛋白在肝癌中的阳性表达率（58.1%）明显高于癌旁组织的阳性表达率（23.3%）。aPKC-iota的表达与Cyclin E的表达、分化程度、肿瘤侵袭程度呈正相关（P < 0.05）。肝癌的分化程度和侵袭性与aPKC-iota的表达有关，aPKC-iota在肝癌的侵袭转移中起着重要作用。(C) 2009爱思唯尔公司版权所有。
Background and objective. Previous studies have indicated that abnormal expression of atypical protein kinase C (aPKC-iota) plays a critical role in occurrence and progression of malignant tumor. This study analyzed the correlation of aPKC-iota with clinicopathology in hepatocarcinoma and Cyclin E and investigated molecular mechanisms of invasion and metastasis of hepatocellular carcinoma.Materials and methods. The expression of the aPKC-iota gene was examined by reverse transcription-polymerase chain reaction in 7 specimens of normal liver tissues and 43 of hepatoma and adjacent tissues. Expression of aPKC-iota and Cyclin E protein was detected using immunohistochemistry and Western blot. Finally, we analyzed the correlation of aPKC-iota with clinicopathologic characteristics and invasion of hepatoma.Results. The expression value (0.844 +/- 0.315) of aPKC-iota gene is obviously higher in hepatoma than the value (0.530 +/- 0.217) in adjacent tissues and the value (0.372 +/- 0.130) in normal tissue (P = 0.009). The positive expression rate (58.1%) of aPKC-iota protein in hepatoma is remarkably higher than the rate (23.3%) of adjacent tissues. The expression of aPKC-iota has a positive correlation with the expression of Cyclin E, differentiation degree, and invasion of tumor (P < 0.05).Conclusions. Differentiation degree and invasion of hepatoma are related to the expression of aPKC-iota, which plays an important role in invasion and metastasis of hepatoma. (C) 2009 Elsevier Inc. All rights reserved.