The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells.
The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells.
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发表时间：
1997-02
期刊：
影响因子：
4.6
通讯作者：
K. Ito;W. Awano;Kazumi Suzuki;Y. Hiromi;D. Yamamoto
中科院分区：
文献类型：
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作者：
K. Ito;W. Awano;Kazumi Suzuki;Y. Hiromi;D. Yamamoto
蘑菇体（MB）是昆虫高级感觉整合和学习的重要中心。为了分析果蝇中MB神经堆的发育和组织，我们使用结合Flippase（flp）/FRT系统和GAL 4/UAS系统的新技术在成年大脑中进行了细胞谱系分析。我们发现，四个蘑菇体神经母细胞（MBNbs）只产生MB的神经元和神经胶质细胞，并且四个MBNb克隆中的每一个都对整个MB结构做出贡献。标记MB细胞的各种亚群的19种GAL 4增强子-陷阱菌株的表达模式揭示了所有四种MBNb谱系中的重叠细胞类型。使用羟基脲的MBNbs的部分消融显示，四个成神经细胞中的每一个自主地产生已知MB亚结构的全部库。
The mushroom body (MB) is an important centre for higher order sensory integration and learning in insects. To analyse the development and organisation of the MB neuropile in Drosophila, we performed cell lineage analysis in the adult brain with a new technique that combines the Flippase (flp)/FRT system and the GAL4/UAS system. We showed that the four mushroom body neuroblasts (MBNbs) give birth exclusively to the neurones and glial cells of the MB, and that each of the four MBNb clones contributes to the entire MB structure. The expression patterns of 19 GAL4 enhancer-trap strains that mark various subsets of MB cells revealed overlapping cell types in all four of the MBNb lineages. Partial ablation of MBNbs using hydroxyurea showed that each of the four neuroblasts autonomously generates the entire repertoire of the known MB substructures.