Volumetric chemical imaging in vivo by a remote-focusing stimulated Raman scattering microscope
Volumetric chemical imaging in vivo by a remote-focusing stimulated Raman scattering microscope
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DOI：
10.1364/oe.404869
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发表时间：
2020-09-28
期刊：
影响因子：
3.8
通讯作者：
Cheng, Ji-Xin
中科院分区：
文献类型：
--
作者：
Lin, Peng;Ni, Hongli;Cheng, Ji-Xin
受激拉曼散射（SRS）显微镜可在环境光下操作，并提供化学选择性，为人类受试者的分子事件成像打开了一个新的窗口，例如通过皮肤过滤局部药物。用于体积SRS成像的典型方法是通过物镜透镜的压电扫描，其经常干扰样品并提供低的轴向扫描速率。为了解决这些挑战，我们已经开发出一种基于可变形反射镜的远程聚焦SRS显微镜，它不仅可以实现高质量的体积化学成像，而无需机械扫描的物镜，但也纠正系统像差的同时。使用远程聚焦SRS显微镜，我们进行了活细胞的体积化学成像，并在真实的时间内捕获局部化学物质进入人体毛孔的动态扩散。(C)根据OSA开放获取出版协议的条款，2020年美国光学学会
Operable under ambient light and providing chemical selectivity, stimulated Raman scattering (SRS) microscopy opens a new window for imaging molecular events on a human subject, such as filtration of topical drugs through the skin. A typical approach for volumetric SRS imaging is through piezo scanning of an objective lens, which often disturbs the sample and offers a low axial scan rate. To address these challenges, we have developed a deformable mirror-based remote-focusing SRS microscope, which not only enables high-quality volumetric chemical imaging without mechanical scanning of the objective but also corrects the system aberrations simultaneously. Using the remote-focusing SRS microscope, we performed volumetric chemical imaging of living cells and captured in real time the dynamic diffusion of topical chemicals into human sweat pores. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement