CYTOGENETIC AND FLUORESCENCE IN-SITU HYBRIDIZATION INVESTIGATION OF RING CHROMOSOMES CHARACTERIZING A SPECIFIC PATHOLOGICAL SUBGROUP OF ADIPOSE-TISSUE TUMORS
CYTOGENETIC AND FLUORESCENCE IN-SITU HYBRIDIZATION INVESTIGATION OF RING CHROMOSOMES CHARACTERIZING A SPECIFIC PATHOLOGICAL SUBGROUP OF ADIPOSE-TISSUE TUMORS
复制标题
DOI：
10.1016/0165-4608(93)90001-3
复制
发表时间：
1993-07-15
影响因子：
--
通讯作者：
VANDENBERGHE, H
中科院分区：
文献类型：
--
作者：
DALCIN, P;KOOLS, P;VANDENBERGHE, H
对184例脂肪组织肿瘤、175例脂肪瘤和9例脂肉瘤（LPS）的细胞遗传学分析显示，在10例具有共同组织学特征（如非典型基质细胞伴或不伴成脂细胞）的病例中，存在环状染色体和/或长标记染色体。在5例中，这似乎是唯一的细胞遗传学异常。用染色体12q13-q15区特异的微克隆文库进行荧光原位杂交（FISH）分析显示，环状和长标记染色体被广泛染色，表明12号染色体这一特定区域的基因序列存在于这些标记染色体中，很可能是以扩增的形式存在。
Cytogenetic analysis of 184 adipose tissue tumors, 175 lipomas, and nine liposarcomas (LPS) showed the presence of a ring chromosome and/or a long marker chromosome in 10 cases with common histologic features such as atypical stromal cells with or without lipoblasts. In five of the cases, this appeared to be the sole cytogenetic abnormality. Fluorescence in situ hybridization (FISH) analysis with a microclone library specific for chromosome region 12q13-q15 showed extensive staining of the ring and long marker chromosomes, indicating that genetic sequences of this particular region of chromosome 12 are present in these marker chromosomes, most likely in an amplified form.