Progression of inflammation during immunodeficient mouse skeletal muscle regeneration
Progression of inflammation during immunodeficient mouse skeletal muscle regeneration
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DOI：
10.1007/s10974-015-9433-1
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发表时间：
2015-12-01
影响因子：
2.7
通讯作者：
Brzoska, Edyta
中科院分区：
文献类型：
--
作者：
Grabowska, Iwona;Mazur, Magdalena A.;Brzoska, Edyta
骨骼肌损伤会引发炎症反应，这对于受损肌纤维降解和卫星细胞激活至关重要。免疫缺陷小鼠经常被用作研究移植的人类干细胞的生肌潜力的模型。因此，阐明这种模型是否真实反映生理条件下发生的过程至关重要。为了回答这个问题，我们比较了 BALB/c（即产生所有类型炎症细胞的动物）和 SCID 小鼠的骨骼肌再生。我们的研究结果证明，两种小鼠的肌肉再生初始阶段是相似的。然而，在 SCID 小鼠肌肉再生中发现单核细胞数量较少。还观察到 BALB/c 和 SCID 肌肉之间 CD14-/CD45+ 和 CD14+/CD45+ 细胞数量的显着差异。此外，我们发现通过 CD68 和 CD163 标记识别的 BALB/c 和 SCID 小鼠肌肉的 M1 和 M2 巨噬细胞水平存在重要差异。因此，我们的数据表明，肌肉再生过程中炎症反应的差异并未转化为肌肉再生的显着改变。
The skeletal muscle injury triggers the inflammatory response which is crucial for damaged muscle fiber degradation and satellite cell activation. Immunodeficient mice are often used as a model to study the myogenic potential of transplanted human stem cells. Therefore, it is crucial to elucidate whether such model truly reflects processes occurring under physiological conditions. To answer this question we compared skeletal muscle regeneration of BALB/c, i.e. animals producing all types of inflammatory cells, and SCID mice. Results of our study documented that initial stages of muscles regeneration in both strains of mice were comparable. However, lower number of mononucleated cells was noticed in regenerating SCID mouse muscles. Significant differences in the number of CD14-/CD45+ and CD14+/CD45+ cells between BALB/c and SCID muscles were also observed. In addition, we found important differences in M1 and M2 macrophage levels of BALB/c and SCID mouse muscles identified by CD68 and CD163 markers. Thus, our data show that differences in inflammatory response during muscle regeneration, were not translated into significant modifications in muscle regeneration.