Anti-inflammatory effects of Melaleuca alternifolia essential oil on human polymorphonuclear neutrophils and monocytes
Anti-inflammatory effects of Melaleuca alternifolia essential oil on human polymorphonuclear neutrophils and monocytes
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DOI：
10.1080/1071576042000220247
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发表时间：
2004-08-01
影响因子：
3.3
通讯作者：
Guillot, J
中科院分区：
文献类型：
--
作者：
Caldefie-Chézet, F;Guerry, M;Guillot, J
目的和设计：互叶白千层挥发油(EO)的抗真菌和杀菌作用已被证实，但其抗炎和抗氧化作用尚不清楚。在这项研究中，我们在体外研究了整个互花米草对人类白细胞(单核细胞和中性粒细胞)氧化反应即产生活性氧(ROS)的可能调节作用。方法：健康志愿者(n=7)通过溶血性休克从红细胞中分离出全血白细胞，与互花链霉菌(0-0.1%)孵育30min，用流式细胞仪测定其在细胞刺激或不刺激的情况下产生的ROS。我们比较了佛波酯(PMA)、甲硫氨酰-亮氨酰苯丙氨酸(FMLP)和优化酵母多糖(OZ)三种不同的刺激剂对刺激ROS产生的转导途径的影响。结果：通过Kruskall-Wallis检验，0.1%的互花米草EO直接刺激PMN产生ROS(X 8.7vs.0%EO，p<；0.05)，并增加单核细胞产生的ROS。无论使用哪种刺激剂(PMA、fMLP或OZ)，互作木霉都能在0.1%的稀释度内减少中性粒细胞和单核细胞产生的ROS，对PMN的抑制作用更强。结论：互叶木霉可能是循环白细胞杀菌作用的直接活性介体，并可能通过抗氧化和清除自由基的活性来保护机体免受过量ROS的伤害。
Objective and design: The fungicidal and bactericidal actions of the essential oil (EO) of Melaleuca alternifolia seem well established, but their anti-inflammatory and anti-oxidative effects remain unclear. In this study, we investigated in vitro the possible role of whole M. alternifolia EO as a modulator of the oxidative response, i.e. reactive oxygen species (ROS) production, of leukocytes (monocytes and polymorphonuclear neutrophils (PMNs)) in humans.Methods: Whole blood leukocytes from healthy human volunteers (n = 7), isolated from erythrocytes by haemolytic shock, were incubated for 30 min with M. alternifolia EO (0-0.1%) to determine their ROS production by flow cytometry with or without stimulation of cells. We compared the effects of 3 different stimulating agents acting differently on transductional pathways to stimulate the ROS production: a phorbol ester (PMA), formylmethionyl-leucyl-phenylalanine (fMLP) and opsonised zymosan (OZ).Results: As attested by the Kruskall-Wallis test, M. alternifolia EO at 0.1% directly stimulated ROS production by PMNs ( X 8.7 vs. 0% EO, p < 0.05) and increased the intracellular ROS produced by monocytes. Whichever the stimulating agent used (PMA, fMLP or OZ), M. alternifolia EO decreased the intracellular ROS production at the dilution of 0.1% by PMNs and monocytes, more so with PMNs.Conclusion: M. alternifolia EO may be both a direct active mediator of the bactericidal action of the circulating leukocytes and may be efficient in protecting the organism from an excess of ROS, through an anti-oxidant and radical scavenging activity.