Proteomics of Colwellia psychrerythraea at subzero temperatures - a life with limited movement, flexible membranes and vital DNA repair
Proteomics of Colwellia psychrerythraea at subzero temperatures - a life with limited movement, flexible membranes and vital DNA repair
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DOI：
10.1111/1462-2920.12691
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发表时间：
2015-07-01
影响因子：
5.1
通讯作者：
Junge, Karen
中科院分区：
文献类型：
--
作者：
Nunn, Brook L.;Slattery, Krystal V.;Junge, Karen
允许嗜冷细菌在零度以下温度下保持代谢活性的机制是由其蛋白质的形式和功能引起的。我们提出了第一个蛋白质组学证据的生理变化的海洋嗜冷Colwellia psychrerythraea 34 H（Cp 34 H）后，暴露于零度以下的温度（-1，和-10摄氏度的冰）通过8周。在不同处理之间比较蛋白质丰度，以了解温度和时间在细胞内独立和联合地过渡到冰中并保持在冰中的影响。平行的[3 H]-亮氨酸和[3 H]-胸苷孵育表明活性蛋白质和DNA合成至-10 ℃。基于质谱的蛋白质组学在四种实验处理中鉴定了1763种蛋白质。发现参与渗透调节和聚合物分泌的蛋白质在所有处理中组成性存在，表明它们是0 ℃以下代谢成功所必需的。差异丰富的蛋白质组表明，从DNA结合到DNA修复，从运动到趋化性和传感资源的重新分配。铁和氮代谢，细胞膜结构，蛋白质合成和折叠的变化也被揭示。通过阐明在冰中生活期间的重要策略，这项研究为适应寒冷的海洋生物在其栖息地维持细胞功能的广泛分子适应提供了新的见解。
The mechanisms that allow psychrophilic bacteria to remain metabolically active at subzero temperatures result from form and function of their proteins. We present first proteomic evidence of physiological changes of the marine psychrophile Colwellia psychrerythraea 34H (Cp34H) after exposure to subzero temperatures (-1, and -10 degrees C in ice) through 8 weeks. Protein abundance was compared between different treatments to understand the effects of temperature and time, independently and jointly, within cells transitioning to, and being maintained in ice. Parallel [3H]-leucine and [3H]-thymidine incubations indicated active protein and DNA synthesis to -10 degrees C. Mass spectrometry-based proteomics identified 1763 proteins across four experimental treatments. Proteins involved in osmolyte regulation and polymer secretion were found constitutively present across all treatments, suggesting that they are required for metabolic success below 0 degrees C. Differentially abundant protein groups indicated a reallocation of resources from DNA binding to DNA repair and from motility to chemo-taxis and sensing. Changes to iron and nitrogen metabolism, cellular membrane structures, and protein synthesis and folding were also revealed. By elucidating vital strategies during life in ice, this study provides novel insight into the extensive molecular adaptations that occur in cold-adapted marine organisms to sustain cellular function in their habitat.