Expressions of Uroplakins in the Mouse Urinary Bladder with Cyclophosphamide-Induced Cystitis
Expressions of Uroplakins in the Mouse Urinary Bladder with Cyclophosphamide-Induced Cystitis
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DOI：
10.3346/jkms.2009.24.4.684
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发表时间：
2009-08-01
影响因子：
4.5
通讯作者：
Lee, Gilho
中科院分区：
文献类型：
--
作者：
Choi, Seong Hoo;Byun, Youngmin;Lee, Gilho
尽管尿斑蛋白（UPs）被认为是对有毒物质的强大保护屏障，但环磷酰胺（CP）会引起广泛的膀胱炎。我们研究了CP诱导的小鼠膀胱炎中UPs的表达。共27只ICR雌性小鼠接受200 mg CP/kg单次腹膜内注射。在注射后12、24和72小时依次处死9只CP处理小鼠和6只对照小鼠。广泛的膀胱炎和膀胱重量增加。这些均在注射后12小时内达到峰值，此后趋于下降。所有的UPs mRNA的水平，免疫印迹研究中UP II和III的蛋白表达，以及免疫定位研究中UP III的表达在12小时内受到最大抑制;这在CP注射后24小时部分恢复，并在72小时完全恢复。总之，CP降低了UPs的表达。UPs mRNA和蛋白的减少是时间依赖性的，并且在CP注射后12小时内达到峰值。然而，损伤迅速修复24 hr. This研究表明，一个动态的过程中，广泛的减少和快速恢复，为CP注射后的小鼠膀胱UPs的表达。
Even though uroplakins (UPs) are believed to serve a strong protective barrier against toxic materials, cyclophosphamide (CP) causes extensive cystitis. We investigated the expression of UPs in the urothelium in CP induced mouse cystitis. A total of 27 ICR female mice received a single intraperitoneal injection of 200 mg CP/kg. Nine CP-treated mice and 6 controls were sequentially killed at 12, 24, and 72 hr post injection. Extensive cystitis and an increased vesical weight were seen. These all peaked within 12 hr post injection and they tended to decrease thereafter. The level of all the UPs mRNA, the protein expressions of UP II and III on immunoblotting study, and the expression of UP III on immunolocalization study were maximally suppressed within 12 hr; this partially recovered at 24 hr, and this completely recovered at 72 hr post CP injection. In conclusion, CP reduced the expression of UPs. The reduction of the UPs mRNA and protein was time dependent, and this peaked within 12 hr after CP injection. However, the damage was rapidly repaired within 24 hr. This study demonstrates a dynamic process, an extensive reduction and rapid recovery, for the UPs expression of the mouse urinary bladder after CP injection.