IN-VITRO EVOLUTION OF NEW RIBOZYMES WITH POLYNUCLEOTIDE KINASE-ACTIVITY
IN-VITRO EVOLUTION OF NEW RIBOZYMES WITH POLYNUCLEOTIDE KINASE-ACTIVITY
复制标题
DOI：
10.1038/371031a0
复制
发表时间：
1994-09-01
期刊：
影响因子：
64.8
通讯作者：
SZOSTAK, JW
中科院分区：
文献类型：
--
作者：
LORSCH, JR;SZOSTAK, JW
我们已经从由随机序列区域侧翼的ATP结合结构域组成的RNA分子池中分离出大量的多核苷酸激酶核酶。不同种类的激酶催化ATP-γ S的γ-硫代磷酸转移到5 ′-羟基或内部2 ′-羟基。一类的工程化版本能够催化硫代磷酸从ATP-γ S转移到外源寡核糖核苷酸底物的5 '-羟基，具有多重周转，因此充当真正的酶。
We have isolated a large number of polynucleotide kinase ribozymes from a pool of RNA molecules consisting of an ATP-binding domain flanked by regions of random sequence. Different classes of kinases catalyse the transfer of the gamma-thiophosphate of ATP-gamma S to the 5'-hydroxyl or to internal 2'-hydroxyls. An engineered version of one class is able to catalyse the transfer of thiophosphate from ATP-gamma S to the 5'-hydroxyl of an exogenous oligoribonucleotide substrate with multiple turnover, thus acting as a true enzyme.