Genome-wide analysis on Chlamydomonas reinhardtii reveals the impact of hydrogen peroxide on protein stress responses and overlap with other stress transcriptomes.
Genome-wide analysis on Chlamydomonas reinhardtii reveals the impact of hydrogen peroxide on protein stress responses and overlap with other stress transcriptomes.
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DOI：
10.1111/tpj.13053
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Merchant SS
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文献类型：
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作者：
Blaby IK;Blaby-Haas CE;Pérez-Pérez ME;Schmollinger S;Fitz-Gibbon S;Lemaire SD;Merchant SS
活性氧（ROS）是由所有需氧生物产生的，并有可能对所有需氧生物造成损害。在光合生物中，它们是叶绿体和叶绿体中电子转移不可避免的副产品。我们采用参考单细胞绿色衣藻，莱茵衣藻，确定过氧化氢对基因表达的影响，通过监测转录组的变化，在一个时间进程的实验。比较转录组从细胞采样前立即加入过氧化氢，0.5和1小时，随后发现1278差异丰富的成绩单。在丰度增加的那些转录本中，许多编码参与ROS解毒、蛋白质降解和应激反应的蛋白质，而在那些丰度减少的转录本中，编码参与光合作用和中心碳代谢的蛋白质。除了这些转录组学的调整，我们观察到，H2O2除了是由总细胞内谷胱甘肽池的积累和氧化，并减少光合O2输出。此外，我们分析了我们的转录本丰度变化的背景下，响应单线态O2（O2*），并与我们的H2O2诱导的转录本昼夜转录组，在那里我们证明了丰富的H2O2诱导的转录本早在光相，晚在光相和2小时前光。在此基础上，在这项工作中突出显示的几个基因可能涉及以前未发现的压力补救途径或适应反应。
Reactive oxygen species (ROS) are produced by and have the potential to be damaging to all aerobic organisms. In photosynthetic organisms, they are an unavoidable byproduct of electron transfer in both the chloroplast and mitochondrion. We employ the reference unicellular green alga, Chlamydomonas reinhardtii, to identify the effect of H2O2 on gene expression by monitoring the transcriptome changes in a timecourse experiment. Comparison of transcriptomes from cells sampled immediately prior to addition of H2O2, and 0.5 and 1 h subsequently revealed 1278 differentially abundant transcripts. Of those transcripts that increase in abundance, many encode proteins involved in ROS detoxification, protein degradation and stress-responses, whereas among those that decrease are transcripts encoding proteins involved in photosynthesis and central carbon metabolism. In addition to these transcriptomic adjustments, we observe that H2O2 addition is followed by an accumulation and oxidation of the total intracellular glutathione pool, and a decrease in photosynthetic O2 output. Additionally, we analyze our transcriptomes in the context of transcript abundance changes in response to singlet O2 (O2*), and relate our H2O2-induced transcripts to a diurnal transcriptome, where we demonstrate enrichments of H2O2-induced transcripts early in the light phase, late in the light phase and 2 h prior to light. On this basis several genes that are highlighted in this work may be involved in previously undiscovered stress remediation pathways or acclimation responses.