Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes
Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes
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DOI：
10.1021/ac001454h
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发表时间：
2001-08-15
影响因子：
7.4
通讯作者：
Zheng, WX
中科院分区：
文献类型：
--
作者：
Liu, SP;Luo, HQ;Zheng, WX
在近中性介质中，肝素与一些碱性二苯基萘甲烷染料（例如维多利亚蓝 4R (VB4R)、维多利亚蓝 B (VBB) 或夜蓝 (NB)）的组合可导致共振显着增强。 VB4R、VBB 和 NB 的瑞利散射 (RRS) 及其最大散射波长 lambda (max) 分别出现在 523、534 和 540 nm 处。研究了肝素-染料复合物的RRS光谱特征、影响因素以及这些反应的最佳条件。对于所有系统，RRS 强度与肝素浓度成正比，范围约为 0 至 0.4 微克/毫升。该方法具有高灵敏度，VB4R系统肝素的检测限为3.35 ng/mL，VBB系统为6.62 ng/mL，NB系统为6.29 ng/mL。由于VB4R系统最为灵敏，因此以VB4R系统为例研究该方法的选择性。建立了一种基于RRS技术测定痕量肝素的新方法。此外，还初步讨论了RRS增强的原因以及肝素染料复合物的RRS光谱特性与其吸收光谱之间的关系。
In a near-neutral medium, a combination of heparin with some basic diphenyl naphthylmethane dyes such as victoria blue 4R (VB4R), victoria blue B (VBB), or night blue (NB) can result in a significant enhancement of resonance. Rayleigh scattering (RRS) and their maximum scattering wavelengths lambda (max) appear at 523, 534, and 540 nm for VB4R, VBB, and NB, respectively. The characteristics of RRS spectra of the heparin-dye complexes, the influencing factors, and the optimum conditions of these reactions have been investigated. The RRS intensity is directly proportional to the concentration of heparin in the range of similar to0 to 0.4 mug/mL for all systems. The methods exhibit high sensitivities, and the detection limits for heparin are 3.35 ng/mL for the VB4R system, 6.62 ng/mL for the VBB system, and 6.29 ng/mL for the NB system. Because the VB4R system is the most sensitive, it was taken as an example to study the selectivity of the method. A new method for the determination of trace amounts of heparin based on RRS technique has been developed. Moreover, the enhancement reasons of RRS and the relationship between RRS spectral characteristics of the heparin-dye complex and its absorption spectra have been primarily discussed.