Control genes in reverse transcriptase-polymerase chain reaction assays
Control genes in reverse transcriptase-polymerase chain reaction assays
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DOI：
10.1038/sj.leu.2400711
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发表时间：
1997-06
期刊：
影响因子：
11.4
通讯作者：
V. Kidd;T. Lion
中科院分区：
文献类型：
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作者：
V. Kidd;T. Lion
在这个问题上，白血病提出了上述辩论的第一个贡献。这些评论解决了Kidd博士和Lion博士在早期《白血病》杂志上发表的开场评论中讨论的与RT-PCR对照主题有关的事实和问题，并在以下页面中转载。由V Kidd和T Lion提出的有争议的观点为这场辩论提供了动力，这场辩论是在RTPCR技术的使用变得广泛传播的时候进行的，这使得确保所涉及的实验室提供可靠的结果变得越来越重要。在这方面，采用适当的控制措施无疑是关键问题之一。我们希望，关于这一挑衅性议题的辩论将导致达成共识，使我们能够发布普遍接受的准则。然而，不会强迫达成共识，而且据了解，准则需要修订。NMB，编辑
In this issue, Leukemia presents the first contributions to the above-mentioned debate. The commentaries address facts and questions pertaining to the topic of RT-PCR controls discussed by Dr Kidd and Dr Lion in their opening comments published in earlier issues of Leukemia and reprinted in the following pages. The controversial viewpoints put forward by V Kidd and T Lion provided the stimulus for this debate, which is being pursued at a time when the use of RTPCR techniques is becoming widely disseminated, making it increasingly important to ensure that the laboratories involved provide reliable results. In this context, the use of adequate controls is certainly one of the critical issues. We hope that the debate on this provocative topic will lead to a consensus enabling us to issue generally acceptable guidelines. However, a consensus will not be forced, and it is understood that guidelines need to be revised. NMB, Editor