C6 glioma-astrocytoma cell and fetal astrocyte migration into artificial basement membrane: a permissive substrate for neural tumors but not fetal astrocytes.
C6 glioma-astrocytoma cell and fetal astrocyte migration into artificial basement membrane: a permissive substrate for neural tumors but not fetal astrocytes.
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C6 神经胶质瘤-星形细胞瘤细胞和胎儿星形胶质细胞迁移到人工基底膜：神经肿瘤但不是胎儿星形胶质细胞的允许基质。
DOI：
10.1097/00006123-199105000-00003
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发表时间：
1991
期刊：
影响因子：
4.8
通讯作者：
Goldberg,WJ
中科院分区：
文献类型：
--
作者：
Bernstein,JJ;LawsJr,ER;Levine,KV;Wood,LR;Tadvalkar,G;Goldberg,WJ
皮质同种移植的C6胶质瘤-星形细胞瘤细胞既作为团块侵入宿主脑，又作为单个细胞迁移。与此相反，胎儿星形胶质细胞来源于同种移植的胎儿皮质的整片迁移仅作为单个细胞在整个大脑的大鼠，但不能入侵。我们的实验探讨了培养的纯化的胎儿星形胶质细胞和C6细胞的迁移能力（超过7天）后接种106细胞的水合人工基底膜晶片（Matrigel）。人工基底膜片不是培养胎儿星形胶质细胞生长的合适基质。相比之下，C6细胞作为单个细胞从晶片表面迁移到基底中。单个C6细胞在前4天迁移1.8 mm，然后停止迁移。在消化管的底部观察到C6细胞，消化管从晶片表面延伸并向晶片表面开放。在第3天，观察到在每个管的基部的每个C6细胞周围形成微袋。到第7天，观察到的大多数囊袋较大，含有几个C6细胞。这些多细胞群似乎是肿瘤块的祖细胞。这些数据表明，C6胶质瘤-星形细胞瘤细胞，其在体内似乎是多形性胶质母细胞瘤的模型，主要作为单个细胞迁移通过人工基底膜，其次形成肿瘤块。祖细胞肿瘤块通过单个C6细胞微袋的聚结或单个微袋中单个细胞的分裂形成。（神经外科28：652-658，1991）
: Cortically homografted C6 glioma-astrocytoma cells both invade the rat host brain as a mass and migrate as individual cells. In contrast, fetal astrocytes derived from homografted whole pieces of fetal cortex migrate only as individual cells throughout the brain of the rat but are not capable of invasion. Our experiments explored the migratory capacity (over 7 days) of cultured purified fetal astrocytes and C6 cells after seeding 106 cells on a hydrated artificial basement membrane wafer (Matrigel). The artificial basement membrane wafer was not a suitable substrate for the growth of cultured fetal astrocytes. In contrast, C6 cells migrated as individual cells from the surface of the wafer into the substrate. Individual C6 cells migrated 1.8 mm in the first 4 days and then ceased migration. The C6 cells were observed at the base of a digestion tube that extended from and was open to the surface of the wafer. At 3 days, micropockets were observed to form around each C6 cell at the base of each tube. By 7 days, the majority of pockets observed were large and contained several C6 cells. These multiple cell groups appeared to be progenitors of tumor masses. These data indicate that C6 glioma-astrocytoma cells, which in vivo appear to be a model for glioblastoma multiforme, primarily migrate as individual cells through artificial basement membrane and secondarily form tumor masses. Progenitor tumor masses form by coalescence of individual C6 cell micropockets or the division of a single cell in an individual micropocket.(Neurosurgery 28: 652-658, 1991)