Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies.
Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies.
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通过一组 38 种单克隆抗体测定人神经母细胞瘤和白血病-淋巴瘤细胞系共有的细胞表面膜抗原。
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发表时间：
1984
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通讯作者：
Jun Minowada
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作者：
Tohru Sugimoto;E. Tatsumi;John T Kemshead;Lawrence Helson;Alexander A. Green;Jun Minowada
本文用36种主要抗造血细胞的鼠源单克隆抗体和2种抗人胎脑的鼠源单克隆抗体，对7种神经母细胞瘤和91种白血病淋巴瘤细胞系的表面膜抗原进行了研究。5株抗造血细胞的单克隆抗体（BA-1、BA-2、DU-ALL-1、J-5和BA-3）与常见的急性淋巴细胞白血病细胞系一致结合，另外2株（MCS-2和OKM-1）与急性成髓细胞-急性单核细胞白血病细胞系一致结合。然而，这7个单克隆抗体也与1-7神经母细胞瘤细胞系反应。所有人神经母细胞瘤细胞系均结合MoAb BA-2和DU-ALL-1。7个株系中有6个与BA-1反应。仅1株神经母细胞瘤细胞系（SJ-N-CG）与J-5和BA-3呈阳性反应，另1株细胞系（SK-N-AS）与MoAb MCS-2和OKM-1呈阳性反应。抗胎脑单克隆抗体（UJ-13 A和UJ-127-11）对所有神经母细胞瘤细胞系均呈高度阳性。相比之下，43个白血病淋巴瘤细胞系中有4个检测到这些抗胎儿脑抗体。B3/25和OKT-9，抗转铁蛋白受体抗体，与所有的造血和神经母细胞瘤细胞系反应。这些结果表明，神经母细胞瘤和造血细胞系具有共同的抗原决定簇，尽管他们不同的胚胎起源。神经母细胞瘤细胞系可根据MoAb确定的表型特征分为亚组。单克隆抗体可能是有用的神经母细胞瘤细胞的表征和分类，已经证明是造血谱系细胞的情况。
The surface membrane antigens of 7 neuroblastoma and 91 leukemia-lymphoma cell lines were studied with the use of a total of 36 murine monoclonal antibodies (MoAb) primarily developed against hematopoietic cells and 2 MoAb developed against human fetal brain. Five of the MoAb against hematopoietic cells (BA-1, BA-2, DU-ALL-1, J-5, and BA-3) consistently bound to common acute lymphoblastic leukemia cell lines, and 2 others (MCS-2 and OKM-1) reacted uniformly with acute myeloblastic-acute monoblastic leukemia cell lines. However, these 7 MoAb also reacted with 1-7 neuroblastoma cell lines. All the human neuroblastoma cell lines bound MoAb BA-2 and DU-ALL-1. Six of the 7 lines reacted with BA-1. Only 1 neuroblastoma cell line (SJ-N-CG) gave positive staining with J-5 and BA-3, and another line (SK-N-AS) bound MoAb MCS-2 and OKM-1. Anti-fetal brain MoAb (UJ-13A and UJ-127-11) were highly positive for all the neuroblastoma cell lines. By contrast, 4 of 43 leukemia-lymphoma cell lines tested bound these anti-fetal brain antibodies. Both B3/25 and OKT-9, anti-transferrin receptor antibodies, reacted with all of the hematopoietic and neuroblastoma cell lines. These results demonstrate that neuroblastoma and hematopoietic cell lines possess common antigenic determinants despite their different embryologic origins. The neuroblastoma cell lines may be classified into subgroups on the basis of phenotype profiles determined by the MoAb. MoAb may be useful in characterization and classification of neuroblastoma cells, as has already proved to be the case for cells of the hematopoietic lineages.